Laccase-mimicking Mn-Cu hybrid nanoflowers for paper-based visual detection of phenolic neurotransmitters and rapid degradation of dyes
- Authors
- Le, Thao Nguyen; Le, Xuan Ai; Tran, Tai Duc; Lee, Kang Jin; Kim, Moon Il
- Issue Date
- Aug-2022
- Publisher
- BMC
- Keywords
- Colorimetric detection; Laccase-mimicking nanozyme; Neurotransmitter detection; Paper microfluidic devices; Protein-free nanoflower
- Citation
- Journal of Nanobiotechnology, v.20, no.1
- Journal Title
- Journal of Nanobiotechnology
- Volume
- 20
- Number
- 1
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85271
- DOI
- 10.1186/s12951-022-01560-0
- ISSN
- 1477-3155
- Abstract
- Background: Laccase-based biosensors are efficient for detecting phenolic compounds. However, the instability and high cost of laccases have hindered their practical utilization.
Results: In this study, we developed hierarchical manganese dioxide-copper phosphate hybrid nanoflowers (H- Mn-Cu NFs) as excellent laccase-mimicking nanozymes. To synthesize the H-Mn-Cu NFs, manganese dioxide nanoflowers (MnO2 NFs) were first synthesized by rapidly reducing potassium permanganate using citric acid. The MnO2 NFs were then functionalized with amine groups, followed by incubation with copper sulfate for three days at room temperature to drive the coordination interaction between the amine moieties and copper ions and to induce anisotropic growth of the petals composed of copper phosphate crystals, consequently yielding H-Mn-Cu NFs. Compared with those of free laccase, at the same mass concentration, H-Mn-Cu NFs exhibited lower K-m (similar to 85%) and considerably higher V-max (similar to 400%), as well as significantly enhanced stability in the ranges of pH, temperature, ionic strength, and incubation periods evaluated. H-Mn-Cu NFs also catalyzed the decolorization of diverse dyes considerably faster than the free laccase. Based on these advantageous features, a paper microfluidic device incorporating H-Mn-Cu NFs was constructed for the convenient visual detection of phenolic neurotransmitters, including dopamine and epinephrine. The device enabled rapid and sensitive quantification of target neurotransmitters using an image acquired using a smartphone.
Conclusions: These results clearly show that H-Mn-Cu NFs could be potential candidates to replace natural laccases for a wide range of applications in biosensing, environmental protection, and biotechnology.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85271)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.