Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics
DC Field | Value | Language |
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dc.contributor.author | De, Anindita | - |
dc.contributor.author | Ko, Young Tag | - |
dc.date.accessioned | 2022-10-07T01:40:06Z | - |
dc.date.available | 2022-10-07T01:40:06Z | - |
dc.date.created | 2022-09-22 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1071-7544 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85623 | - |
dc.description.abstract | Ionizable LNPs are the latest trend in nucleic acid delivery. Microfluidics technology has recently gained interest owing to its rapid mixing, production of nucleic acid-ionizable LNPs, and stability of nucleic acid inside the body. Industrial scale-up, nucleic acid-lipid long-term storage instability, and high production costs prompted scientists to seek alternate solutions to replace microfluidic technology. We proposed a single-pot, organic solvent-free thermocycling technology to efficiently and economically overcome most of the limitations of microfluidic technology. New thermocycling technology needs optimization of process parameters such as sonication duration, cooling-heating cycle, number of thermal cycles, and lipid:aqueous phase ratio to formulate precisely sized particles, effective nucleic acid encapsulation, and better shelf-life stability. Our research led to the formulation of siRNA-ionizable LNPs with particle sizes of 104.2 +/- 34.7 nm and PDI 0.111 +/- 0.109, with 83.3 +/- 4.1% siRNA encapsulation. Thermocycling siRNA-ionizable LNPs had comparable morphological structures with commercialized microfluidics ionizable LNPs imaged by TEM and cryo-TEM. When compared to microfluidics ionizable LNPs, thermocycling siRNA-ionizable LNPs had a longer shelf life at 4 degrees C. Our thermocycling technology showed an effective alternative to microfluidics technology in the production of nucleic acid-ionizable LNPs to meet global demand. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | DRUG DELIVERY | - |
dc.title | Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000839327400001 | - |
dc.identifier.doi | 10.1080/10717544.2022.2108523 | - |
dc.identifier.bibliographicCitation | DRUG DELIVERY, v.29, no.1, pp.2644 - 2657 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85135935835 | - |
dc.citation.endPage | 2657 | - |
dc.citation.startPage | 2644 | - |
dc.citation.title | DRUG DELIVERY | - |
dc.citation.volume | 29 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | De, Anindita | - |
dc.contributor.affiliatedAuthor | Ko, Young Tag | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Thermocycling technology | - |
dc.subject.keywordAuthor | cooling-heating cycle | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | ionizable LNPs | - |
dc.subject.keywordAuthor | LNPs stability | - |
dc.subject.keywordPlus | LONG-TERM STORAGE | - |
dc.subject.keywordPlus | MESSENGER-RNA VACCINE | - |
dc.subject.keywordPlus | LIPID NANOPARTICLES | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | FORMULATION | - |
dc.subject.keywordPlus | EMULSIFICATION | - |
dc.subject.keywordPlus | NANOEMULSIONS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | DNA | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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