In vitro elucidation of antioxidant, antiproliferative, and apoptotic potential of yeast-derived beta-1,3-glucan particles against cervical cancer cells

Abstract

Cancer is the leading cause of mortality worldwide and in particular is the fourth most common cause of mortality in women every year. Conventional treatments for cancer are chemotherapy and radiation therapy, which have various kinds of side effects. Hence, there is a high need to develop alternative, efficient, and safer therapies for cancer treatment. beta-Glucan, a novel polysaccharide isolated from baker's yeast Saccharomyces cerevisiae, shows noteworthy cytotoxicity toward a variety of cancer cell lines in vitro. In this research, we characterized the beta-glucan with high-performance thin-layer chromatography (HPTLC) analysis and found that D-glucose units with beta-1,3 links are the major component of the extracted beta-glucan particles. Fourier transform IR (FTIR) analysis confirmed a beta-(1 -> 3)-linked glucan structure. In vitro cell cytotoxicity was evaluated by MTT with IC50 136 mu g/ml, and therapeutic potential was assessed by various assays using values below and above the IC50. A significant reactive oxygen species (ROS) generation at 50-150 mu g/ml of concentrations indicated the apoptosis of cervical cancer cells. Along with ROS generation, these concentrations were also found to induce morphological changes such as fragmentation in DNA upon staining HeLa cells with DAPI. Mitochondrial membrane potential was significantly reduced after increasing the dose of treatment, assessed with the help of MitoTracker dye. Hence, by all these experimental supports, we observed that beta-glucan has the potential to slow down the growth of cervical cancer cells, and it can be further investigated for unfolding its complete anticancer potential.