Optical imaging of cholylglycine by using liquid crystal droplet patterns on solid surfaces
- Authors
- Wei, Yibin; Jang, Chang-Hyun
- Issue Date
- Feb-2016
- Publisher
- SPRINGER
- Citation
- JOURNAL OF MATERIALS SCIENCE, v.51, no.4, pp.2033 - 2040
- Journal Title
- JOURNAL OF MATERIALS SCIENCE
- Volume
- 51
- Number
- 4
- Start Page
- 2033
- End Page
- 2040
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8583
- DOI
- 10.1007/s10853-015-9513-z
- ISSN
- 0022-2461
- Abstract
- Determining cholylglycine (CG) levels is of great importance in the detection of liver abnormalities. In this study, we present a novel liquid-crystal (LC)-based method assisted with cholylglycine hydrolase (CGH) for the optical detection of CG levels. The detection method is based on the disruption of the orientations of a nematic LC, 4-cyano-4 '-pentylbiphenyl (5CB), doped with dodecyl aldehyde. Aldehyde-doped 5CB droplets were placed on pre-treated glass slides and patterned with solutions of interest. When in contact with a small drop of a CG aqueous solution, bright fan-shaped LC droplet patterns were observed under polarizing optical microscopy, indicating a planar orientation of LC at the aqueous/LC interface. However, aldehyde with short-alkyl chain couples with glycine released from the enzymatic reaction between CG and CGH forming amphiphilic Schiff bases, which display dark cross patterns of LC, suggesting a homeotropic orientation of the LC. This system may offer a highly sensitive and methodologically simple approach to determine CG levels for clinical diagnostics and commercial applications.
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