MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiencyopen access
- Authors
- Umair, Zobia; Baek, Mi-Ock; Song, Jisue; An, Seona; Chon, Seung Joo; Yoon, Mee-Sup
- Issue Date
- Sep-2022
- Publisher
- MDPI
- Keywords
- urinary exosome; micro-RNA; premature ovarian insufficiency; hsa-miR-4516; turner syndrome
- Citation
- CELLS, v.11, no.18
- Journal Title
- CELLS
- Volume
- 11
- Number
- 18
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85866
- DOI
- 10.3390/cells11182797
- ISSN
- 2073-4409
- Abstract
- Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women < 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary-but not in the uterus-of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의학과 > 1. Journal Articles
- 의과대학 > 의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85866)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.