Cytokine-release kinetics of platelet-rich plasma according to various activation protocols
DC Field | Value | Language |
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dc.contributor.author | Roh, Y. H. | - |
dc.contributor.author | Kim, W. | - |
dc.contributor.author | Park, K. U. | - |
dc.contributor.author | Oh, J. H. | - |
dc.date.available | 2020-02-28T02:45:50Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 2046-3758 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8610 | - |
dc.description.abstract | Objectives This study was conducted to evaluate the cytokine-release kinetics of platelet-rich plasma (PRP) according to different activation protocols. Methods Two manual preparation procedures (single-spin (SS) at 900 g for five minutes; double-spin (DS) at 900 g for five minutes and then 1500 g for 15 minutes) were performed for each of 14 healthy subjects. Both preparations were tested for platelet activation by one of three activation protocols: no activation, activation with calcium (Ca) only, or calcium with a low dose (50 IU per 1 ml PRP) of thrombin. Each preparation was divided into four aliquots and incubated for one hour, 24 hours, 72 hours, and seven days. The cytokine-release kinetics were evaluated by assessing PDGF, TGF, VEGF, FGF, IL-1, and MMP-9 concentrations with bead-based sandwich immunoassay. Results The concentration of cytokine released from PRP varied over time and was influenced by various activation protocols. Ca-only activation had a significant effect on the DS PRPs (where the VEGF, FGF, and IL-1 concentrations were sustained) while Ca/thrombin activation had effects on both SS and DS PRPs (where the PDGF and VEGF concentrations were sustained and the TGF and FGF concentrations were short). The IL-1 content showed a significant increase with Ca-only or Ca/thrombin activation while these activations did not increase the MMP-9 concentration. Conclusion The SS and DS methods differed in their effect on cytokine release, and this effect varied among the cytokines analysed. In addition, low dose of thrombin/calcium activation increased the overall cytokine release of the PRP preparations over seven days, relative to that with a calcium-only supplement or non-activation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BRITISH EDITORIAL SOC BONE JOINT SURGERY | - |
dc.relation.isPartOf | BONE & JOINT RESEARCH | - |
dc.subject | RANDOMIZED CONTROLLED-TRIAL | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | DOUBLE-BLIND | - |
dc.subject | CELL-PROLIFERATION | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | MATRIX | - |
dc.subject | THROMBIN | - |
dc.subject | CALCIUM | - |
dc.subject | INJECTIONS | - |
dc.subject | BIOLOGY | - |
dc.title | Cytokine-release kinetics of platelet-rich plasma according to various activation protocols | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000371080400003 | - |
dc.identifier.doi | 10.1302/2046-3758.52.2000540 | - |
dc.identifier.bibliographicCitation | BONE & JOINT RESEARCH, v.5, no.2, pp.37 - 45 | - |
dc.identifier.scopusid | 2-s2.0-84979892380 | - |
dc.citation.endPage | 45 | - |
dc.citation.startPage | 37 | - |
dc.citation.title | BONE & JOINT RESEARCH | - |
dc.citation.volume | 5 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Roh, Y. H. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | platelet-rich plasma | - |
dc.subject.keywordAuthor | cytokine | - |
dc.subject.keywordAuthor | growth factor | - |
dc.subject.keywordAuthor | kinetics | - |
dc.subject.keywordAuthor | activation | - |
dc.subject.keywordAuthor | preparation | - |
dc.subject.keywordPlus | RANDOMIZED CONTROLLED-TRIAL | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | DOUBLE-BLIND | - |
dc.subject.keywordPlus | CELL-PROLIFERATION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordPlus | THROMBIN | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | INJECTIONS | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Orthopedics | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Orthopedics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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