Ocular Distribution and Pharmacokinetics of 8-Oxo-2 '-Deoxyguanosine: A Novel Therapeutic Candidate of Ocular Surface Diseases
- Authors
- Chung, Hyewon; Ha, Yuseung; Kim, Yong Ho; Kim, Dong Hyun; Shin, Dongseong
- Issue Date
- Oct-2022
- Publisher
- MARY ANN LIEBERT, INC
- Keywords
- 8-oxo-dG; RCI001; ocular pharmacokinetics; inflammation
- Citation
- JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS, v.38, no.8, pp.561 - 566
- Journal Title
- JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
- Volume
- 38
- Number
- 8
- Start Page
- 561
- End Page
- 566
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86305
- DOI
- 10.1089/jop.2022.0054
- ISSN
- 1080-7683
- Abstract
- Purpose: This study evaluated the ocular distribution and plasma pharmacokinetics (PKs) of 8-oxo-2 '-deoxyguanosine (8-oxo-dG) in rabbits and rats, respectively.Methods: A test formulation containing radiolabeled [(14) C]8-oxo-dG and unlabeled 8-oxo-dG was ocularly administered to rabbits as a single dose of 1 mg per body and intravenously injected to rats as a single dose of 5 mg/kg. The ocular distribution of [(14) C]8-oxo-dG was evaluated using autoradiography until 48 h postdose. Plasma radioactivity in rabbits and rats was determined until 72 and 168 h, respectively.Results: After ocular instillation, [(14) C]8-oxo-dG distributed into ocular tissues, and high radioactivity concentrations were observed in the ciliary body, conjunctiva, and cornea. The maximum plasma concentration (C-max) and area under the concentration-time curve (AUC(0-t)) were highest in the ciliary body and conjunctiva, respectively. In the conjunctiva, cornea, and aqueous humor, time to reach C-max (T-max) was 0.5 h, and the half-lives were 11.2, 30.2, and 15.1 h, respectively. The radioactivity of [(14) C]8-oxo-dG in plasma of rabbits displayed a double-peak phenomenon with the second peak considered as C-max (37.9 +/- 3.1 ng eq./mL) occurring 24 h postdose. After systemic exposure of [(14) C]8-oxo-dG in rats, a rapid decline in the initial phase and a terminal half-life of 56.1 +/- 31.3 h were observed.Conclusions: Rapid ocular distribution and high concentrations in anterior ocular tissues with minimal systemic exposure were observed after the ocular instillation of 8-oxo-dG in rabbits. These PK profiles are favorable for the treatment of ocular surface diseases.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.