Phage Conversion for beta-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods
DC Field | Value | Language |
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dc.contributor.author | Lee, Young-Duck | - |
dc.contributor.author | Park, Jong-Hyun | - |
dc.date.available | 2020-02-28T03:41:13Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8639 | - |
dc.description.abstract | Temperate phages have been suggested to carry virulence factors and other lysogenic conversion genes that play important roles in pathogenicity. In this study, phage TEM123 in wild-type Staphylococcus aureus from food sources was analyzed with respect to its morphology, genome sequence, and antibiotic resistance conversion ability. Phage TEM123 from a mitomycin C-induced lysate of S. aureus was isolated from foods. Morphological analysis under a transmission electron microscope revealed that it belonged to the family Siphoviridae. The genome of phage TEM123 consisted of a double-stranded DNA of 43,786 bp with a G+C content of 34.06%. A bioinformatics analysis of the phage genome identified 43 putative open reading frames (ORFs). ORF1 encoded a protein that was nearly identical to the metallo-beta-lactamase enzymes that degrade beta-lactam antibiotics. After transduction to S. aureus with phage TEM123, the metallo-beta-lactamase gene was confirmed in the transductant by PCR and sequencing analyses. In a beta-lactam antibiotic susceptibility test, the transductant was more highly resistant to beta-lactam antibiotics than S. aureus S133. Phage TEM123 might play a role in the transfer of beta-lactam antibiotic resistance determinants in S. aureus. Therefore, we suggest that the prophage of S. aureus with its exotoxin is a risk factor for food safety in the food chain through lateral gene transfer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.subject | ANTIMICROBIAL RESISTANCE | - |
dc.subject | EVOLUTION | - |
dc.subject | STRAINS | - |
dc.title | Phage Conversion for beta-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000370856000007 | - |
dc.identifier.doi | 10.4014/jmb.1508.08042 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.26, no.2, pp.263 - 269 | - |
dc.identifier.kciid | ART002082385 | - |
dc.identifier.scopusid | 2-s2.0-84975704712 | - |
dc.citation.endPage | 269 | - |
dc.citation.startPage | 263 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 26 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Park, Jong-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Genome | - |
dc.subject.keywordAuthor | metallo-beta-lactamase | - |
dc.subject.keywordAuthor | phage conversion | - |
dc.subject.keywordAuthor | Staphylococcus aureus | - |
dc.subject.keywordPlus | ANTIMICROBIAL RESISTANCE | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | STRAINS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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