Phage Conversion for beta-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods
- Authors
- Lee, Young-Duck; Park, Jong-Hyun
- Issue Date
- Feb-2016
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Genome; metallo-beta-lactamase; phage conversion; Staphylococcus aureus
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.26, no.2, pp.263 - 269
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 26
- Number
- 2
- Start Page
- 263
- End Page
- 269
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8639
- DOI
- 10.4014/jmb.1508.08042
- ISSN
- 1017-7825
- Abstract
- Temperate phages have been suggested to carry virulence factors and other lysogenic conversion genes that play important roles in pathogenicity. In this study, phage TEM123 in wild-type Staphylococcus aureus from food sources was analyzed with respect to its morphology, genome sequence, and antibiotic resistance conversion ability. Phage TEM123 from a mitomycin C-induced lysate of S. aureus was isolated from foods. Morphological analysis under a transmission electron microscope revealed that it belonged to the family Siphoviridae. The genome of phage TEM123 consisted of a double-stranded DNA of 43,786 bp with a G+C content of 34.06%. A bioinformatics analysis of the phage genome identified 43 putative open reading frames (ORFs). ORF1 encoded a protein that was nearly identical to the metallo-beta-lactamase enzymes that degrade beta-lactam antibiotics. After transduction to S. aureus with phage TEM123, the metallo-beta-lactamase gene was confirmed in the transductant by PCR and sequencing analyses. In a beta-lactam antibiotic susceptibility test, the transductant was more highly resistant to beta-lactam antibiotics than S. aureus S133. Phage TEM123 might play a role in the transfer of beta-lactam antibiotic resistance determinants in S. aureus. Therefore, we suggest that the prophage of S. aureus with its exotoxin is a risk factor for food safety in the food chain through lateral gene transfer.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8639)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.