Anti-Melanogenic Potentials of Nanoparticles from Calli of Resveratrol-Enriched Rice against UVB-Induced Hyperpigmentation in Guinea Pig Skin
- Authors
- Lee, Taek Hwan; Kang, Ji Hee; Seo, Jae Ok; Baek, So-Hyeon; Moh, Sang Hyun; Chae, Jae Kyoung; Park, Yong Un; Ko, Young Tag; Kim, Sun Yeou
- Issue Date
- 1-Jan-2016
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Keywords
- Plant tissue culture; High-pressure homogenization; Melanogenesis; Resveratrol-enriched rice; Nanoparticles
- Citation
- BIOMOLECULES & THERAPEUTICS, v.24, no.1, pp.85 - 93
- Journal Title
- BIOMOLECULES & THERAPEUTICS
- Volume
- 24
- Number
- 1
- Start Page
- 85
- End Page
- 93
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8681
- DOI
- 10.4062/biomolther.2015.165
- ISSN
- 1976-9148
- Abstract
- We already reported that genetically engineered resveratrol-enriched rice (RR) showed to down-regulate skin melanogenesis. To be developed to increase the bioactivity of RR using calli from plants, RR was adopted for mass production using plant tissue culture technologies. In addition, high-pressure homogenization (HPH) was used to increase the biocompatibility and penetration of the calli from RR into the skin. We aimed to develop anti-melanogenic agents incorporating calli of RR (cRR) and nanoparticles by high-pressure homogenization, examining the synergistic effects on the inhibition of UVB-induced hyperpigmentation. Depigmentation was observed following topical application of micro-cRR, nano-calli of normal rice (cNR), and nano-cRR to ultraviolet B (UVB)-stimulated hyperpigmented guinea pig dorsal skin. Colorimetric analysis, tyrosinase immunostaining, and Fontana-Masson staining for UVB-promoted melanin were performed. Nano-cRR inhibited changes in the melanin color index caused by UVB-promoted hyperpigmentation, and demonstrated stronger anti-melanogenic potential than micro-cRR. In epidermal skin, nano-cRR repressed UVB-promoted melanin granules, thereby suppressing hyperpigmentation. The UVB-enhanced, highly expressed tyrosinase in the basal layer of the epidermis was inhibited by nano-cRR more prominently than by micro-cRR and nano-cNR. The anti-melanogenic potency of nano-cRR also depended on pH and particle size. Nano-cRR shows promising potential to regulate skin pigmentation following UV.B exposure.
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