Enzyme-based fluorometric biosensor-based on coffee waste-derived carbon dots modified with APBA and NADP+ cofactor for selective dual detection of γ-aminobutyric acid in in vitro and in vivo models
DC Field | Value | Language |
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dc.contributor.author | Sangubotla, R. | - |
dc.contributor.author | Kim, Jongsung | - |
dc.date.accessioned | 2023-02-03T02:40:06Z | - |
dc.date.available | 2023-02-03T02:40:06Z | - |
dc.date.issued | 2023-02 | - |
dc.identifier.issn | 0272-8842 | - |
dc.identifier.issn | 1873-3956 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86811 | - |
dc.description.abstract | An enzyme-based fluorescent sensor was developed by adapting carbon dots derived from coffee waste to detect inhibitory neurotransmitters such as γ-aminobutyric acid (GABA). In this study, CDs were synthesized from biomass-derived coffee waste by the facile hydrothermal reaction (i.e., C-CDs). A C-CDs-based enzyme-based sensor was then developed using 3-aminophenyl boronic acid (APBA) and nicotinamide adenine dinucleotide phosphate (NADP+) cofactors (i.e., C-CANs). As a result of the addition of the GABase enzyme, the resulting blue-fluorescent C-CANs were effective in detecting GABA. This sensor is capable of sensing GABA in the range of 0–20 μM with a detection limit of 95.09 nM. A distinguished fluorescence quenching was observed in human neuroblastoma (SH-SY5Y) cells where probe (C-CANs-GABase) was able to detect GABA intracellularly. Zebrafish larvae were used to study the sensing potentials of the developed probe against GABA at different concentrations (10 and 20 μM). A validation study was conducted on real samples such as human serum, which showed high recovery values between 97 and 105.6%. © 2022 Elsevier Ltd and Techna Group S.r.l. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Enzyme-based fluorometric biosensor-based on coffee waste-derived carbon dots modified with APBA and NADP+ cofactor for selective dual detection of γ-aminobutyric acid in in vitro and in vivo models | - |
dc.type | Article | - |
dc.identifier.wosid | 000914707800001 | - |
dc.identifier.doi | 10.1016/j.ceramint.2022.09.321 | - |
dc.identifier.bibliographicCitation | Ceramics International, v.49, no.3, pp 4356 - 4364 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85139371701 | - |
dc.citation.endPage | 4364 | - |
dc.citation.startPage | 4356 | - |
dc.citation.title | Ceramics International | - |
dc.citation.volume | 49 | - |
dc.citation.number | 3 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Coffee waste-derived carbon dots | - |
dc.subject.keywordAuthor | Enzyme-based sensor | - |
dc.subject.keywordAuthor | Human serum | - |
dc.subject.keywordAuthor | Intracellular sensing | - |
dc.subject.keywordAuthor | Zebrafish | - |
dc.subject.keywordAuthor | γ-aminobutyric acid | - |
dc.subject.keywordPlus | GRAPHENE QUANTUM DOTS | - |
dc.subject.keywordPlus | FACILE SYNTHESIS | - |
dc.subject.keywordPlus | GABA | - |
dc.subject.keywordPlus | ELECTRODE | - |
dc.subject.keywordPlus | GREEN | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | EMISSION | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Ceramics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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