Enzyme-based fluorometric biosensor-based on coffee waste-derived carbon dots modified with APBA and NADP+ cofactor for selective dual detection of γ-aminobutyric acid in in vitro and in vivo models
- Authors
- Sangubotla, R.; Kim, Jongsung
- Issue Date
- Feb-2023
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Coffee waste-derived carbon dots; Enzyme-based sensor; Human serum; Intracellular sensing; Zebrafish; γ-aminobutyric acid
- Citation
- Ceramics International, v.49, no.3, pp 4356 - 4364
- Pages
- 9
- Journal Title
- Ceramics International
- Volume
- 49
- Number
- 3
- Start Page
- 4356
- End Page
- 4364
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86811
- DOI
- 10.1016/j.ceramint.2022.09.321
- ISSN
- 0272-8842
1873-3956
- Abstract
- An enzyme-based fluorescent sensor was developed by adapting carbon dots derived from coffee waste to detect inhibitory neurotransmitters such as γ-aminobutyric acid (GABA). In this study, CDs were synthesized from biomass-derived coffee waste by the facile hydrothermal reaction (i.e., C-CDs). A C-CDs-based enzyme-based sensor was then developed using 3-aminophenyl boronic acid (APBA) and nicotinamide adenine dinucleotide phosphate (NADP+) cofactors (i.e., C-CANs). As a result of the addition of the GABase enzyme, the resulting blue-fluorescent C-CANs were effective in detecting GABA. This sensor is capable of sensing GABA in the range of 0–20 μM with a detection limit of 95.09 nM. A distinguished fluorescence quenching was observed in human neuroblastoma (SH-SY5Y) cells where probe (C-CANs-GABase) was able to detect GABA intracellularly. Zebrafish larvae were used to study the sensing potentials of the developed probe against GABA at different concentrations (10 and 20 μM). A validation study was conducted on real samples such as human serum, which showed high recovery values between 97 and 105.6%. © 2022 Elsevier Ltd and Techna Group S.r.l.
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