Ultrafiltrated fraction of korean red ginseng extract improves memory impairment of tg2576 mice via inhibition of soluble AB production and acetylcholinesterase activity
DC Field | Value | Language |
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dc.contributor.author | Shin, K.Y. | - |
dc.contributor.author | Won, B.Y. | - |
dc.contributor.author | Ha, H.J. | - |
dc.contributor.author | Yun, Y.S. | - |
dc.contributor.author | Chang, K.-A. | - |
dc.contributor.author | Lee, H.G. | - |
dc.contributor.author | Oh, M.H. | - |
dc.contributor.author | Lee, H. | - |
dc.contributor.author | Park, Y.-S. | - |
dc.contributor.author | Park, J.D. | - |
dc.contributor.author | Pyo, M.K. | - |
dc.date.available | 2020-02-28T03:41:53Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 0975-1491 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8683 | - |
dc.description.abstract | Objective: The goal of this study was to research for an effective fraction on memory improvement of Korean red ginseng. Methods: In this study, 80 % ethanol red ginseng extract (RE) was divided into inner fluid (REUI) and outer fluid (REUO) by the ultrafiltration and then REUO was further separated into four fractions namely, REUO-00, REUO-30, REUO-50 and REUO-70, respectively, by Diaion HP-20 column chromatography. Results: REUO has protected more significantly the H2O2-induced SHSY-5Y cell death than REUI. Interestingly, the hydrophobic parts of the REUO (REUO-EtOHs) such as REUO-30,-50 and-70 decreased more significantly the H2O2-induced cell death than its hydrophilic part (REUO-00) in a dose-dependent manner. Then, we focused on the activity of a candidate for cholinergic functions, because memory deficits of neurodegenerative diseases are closely associated with cholinergic dysfunctions. The REUO-EtOHs (1.25 mg/ml) inhibited the activity of the acetylcholinesterase and its half maximal inhibitory concentration (IC50) was about 2.358 mg/ml. Additionally, we investigated whether the intake of the REUO (50 mg/kg/d) during 12 w could improve memory impairment of 12-month old Tg2576 mice and decrease total soluble amyloid-β (Aβ) proteins in the mouse brain cortex. The REUO alleviated significantly the memory impairment and successfully reduced the levels of the soluble Aβ proteins in the mouse cortex. Conclusion: We finally suggest that the REUO, including majorly its hydrophobic part that may be considered as more effective for memory improvement, will be highly considered as valuable candidate for the memory-enhancing ingredients against cholinergic dysfunctions and cognitive impairments of neurodegenerative diseases including Alzheimer’s disease. © 2016 The Authors. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Innovare Academics Sciences Pvt. Ltd | - |
dc.relation.isPartOf | International Journal of Pharmacy and Pharmaceutical Sciences | - |
dc.title | Ultrafiltrated fraction of korean red ginseng extract improves memory impairment of tg2576 mice via inhibition of soluble AB production and acetylcholinesterase activity | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | International Journal of Pharmacy and Pharmaceutical Sciences, v.8, no.4, pp.272 - 277 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84962170713 | - |
dc.citation.endPage | 277 | - |
dc.citation.startPage | 272 | - |
dc.citation.title | International Journal of Pharmacy and Pharmaceutical Sciences | - |
dc.citation.volume | 8 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Chang, K.-A. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Acetylcholinesterase | - |
dc.subject.keywordAuthor | Alzheimer’s disease | - |
dc.subject.keywordAuthor | Ginseng | - |
dc.subject.keywordAuthor | Memory | - |
dc.subject.keywordAuthor | Ultrafiltration | - |
dc.subject.keywordPlus | acetylcholinesterase | - |
dc.subject.keywordPlus | amyloid beta protein | - |
dc.subject.keywordPlus | Artemisia iwayomogi extract | - |
dc.subject.keywordPlus | dehydroevodiamine | - |
dc.subject.keywordPlus | ginseng extract | - |
dc.subject.keywordPlus | ginsenoside | - |
dc.subject.keywordPlus | hydrogen peroxide | - |
dc.subject.keywordPlus | plant extract | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | animal experiment | - |
dc.subject.keywordPlus | animal model | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | Artemisia iwayomogi | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | brain cortex | - |
dc.subject.keywordPlus | cell death | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | cytotoxicity | - |
dc.subject.keywordPlus | enzyme activity | - |
dc.subject.keywordPlus | extraction | - |
dc.subject.keywordPlus | ginseng | - |
dc.subject.keywordPlus | high performance liquid chromatography | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | IC50 | - |
dc.subject.keywordPlus | memory disorder | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | passive avoidance test | - |
dc.subject.keywordPlus | ultrafiltration | - |
dc.subject.keywordPlus | Western blotting | - |
dc.description.journalRegisteredClass | scopus | - |
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