Vascularization of iNSC spheroid in a 3D spheroid-on-a-chip platform enhances neural maturationopen access
- Authors
- Shin, Nari; Kim, Youngtaek; Ko, Jihoon; Choi, Soon Won; Hyung, Sujin; Lee, Seung-Eun; Park, Seunghyuk; Song, Jiyoung; Jeon, Noo Li; Kang, Kyung-Sun
- Issue Date
- Feb-2022
- Publisher
- WILEY
- Keywords
- 3D spheroid; apoptosis; differentiation; induced neural stem cell; microfluidic; spheroid-on-a-chip; vascularization
- Citation
- BIOTECHNOLOGY AND BIOENGINEERING, v.119, no.2, pp.566 - 574
- Journal Title
- BIOTECHNOLOGY AND BIOENGINEERING
- Volume
- 119
- Number
- 2
- Start Page
- 566
- End Page
- 574
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87300
- DOI
- 10.1002/bit.27978
- ISSN
- 0006-3592
- Abstract
- In vitro platforms for studying the human brain have been developed, and brain organoids derived from stem cells have been studied. However, current organoid models lack three-dimensional (3D) vascular networks, limiting organoid proliferation, differentiation, and apoptosis. In this study, we created a 3D model of vascularized spheroid cells using an injection-molded microfluidic chip. We cocultured spheroids derived from induced neural stem cells (iNSCs) with perfusable blood vessels. Gene expression analysis and immunostaining revealed that the vascular network greatly enhanced spheroid differentiation and reduced apoptosis. This platform can be used to further study the functional and structural interactions between blood vessels and neural spheroids, and ultimately to simulate brain development and disease.
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Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
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