Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
DC Field | Value | Language |
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dc.contributor.author | Park, Hae Sang | - |
dc.contributor.author | Lee, Ji Seung | - |
dc.contributor.author | Kim, Chang-Beom | - |
dc.contributor.author | Lee, Kwang-Ho | - |
dc.contributor.author | Hong, In-Sun | - |
dc.contributor.author | Jung, Harry | - |
dc.contributor.author | Lee, Hanna | - |
dc.contributor.author | Lee, Young Jin | - |
dc.contributor.author | Ajiteru, Olatunji | - |
dc.contributor.author | Sultan, Md Tipu | - |
dc.contributor.author | Lee, Ok Joo | - |
dc.contributor.author | Kim, Soon Hee | - |
dc.contributor.author | Park, Chan Hum | - |
dc.date.accessioned | 2023-03-28T01:40:33Z | - |
dc.date.available | 2023-03-28T01:40:33Z | - |
dc.date.created | 2022-11-08 | - |
dc.date.issued | 2023-03 | - |
dc.identifier.issn | 2380-6761 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87394 | - |
dc.description.abstract | Herein, we report the first study to create a three-dimensional (3D) bioprinted artificial larynx for whole-laryngeal replacement. Our 3D bio-printed larynx was generated using extrusion-based 3D bioprinter with rabbit's chondrocyte-laden gelatin methacryloyl (GelMA)/glycidyl-methacrylated hyaluronic acid (GMHA) hybrid bioink. We used a polycaprolactone (PCL) outer framework incorporated with pores to achieve the structural strength of printed constructs, as well as to provide a suitable microenvironment to support printed cells. Notably, we established a novel fluidics supply (FS) system that simultaneously supplies basal medium together with a 3D bioprinting process, thereby improving cell survival during the printing process. Our results showed that the FS system enhanced post-printing cell viability, which enabled the generation of a large-scale cell-laden artificial laryngeal framework. Additionally, the incorporation of the PCL outer framework with pores and inner hydrogel provides structural stability and sufficient nutrient/oxygen transport. An animal study confirmed that the transplanted 3D bio-larynx successfully maintained the airway. With further development, our new strategy holds great potential for fabricating human-scale larynxes with in vivo-like biological functions for laryngectomy patients. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | BIOENGINEERING & TRANSLATIONAL MEDICINE | - |
dc.title | Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000870345700001 | - |
dc.identifier.doi | 10.1002/btm2.10423 | - |
dc.identifier.bibliographicCitation | BIOENGINEERING & TRANSLATIONAL MEDICINE, v.8, no.2 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85140262585 | - |
dc.citation.title | BIOENGINEERING & TRANSLATIONAL MEDICINE | - |
dc.citation.volume | 8 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Hong, In-Sun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | artificial larynx | - |
dc.subject.keywordAuthor | laryngectomy | - |
dc.subject.keywordAuthor | three-dimensional bioprinting | - |
dc.subject.keywordAuthor | tissue engineering | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | SCAFFOLD | - |
dc.subject.keywordPlus | CONSTRUCTS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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