Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabricationopen access
- Authors
- Park, Hae Sang; Lee, Ji Seung; Kim, Chang-Beom; Lee, Kwang-Ho; Hong, In-Sun; Jung, Harry; Lee, Hanna; Lee, Young Jin; Ajiteru, Olatunji; Sultan, Md Tipu; Lee, Ok Joo; Kim, Soon Hee; Park, Chan Hum
- Issue Date
- Mar-2023
- Publisher
- WILEY
- Keywords
- artificial larynx; laryngectomy; three-dimensional bioprinting; tissue engineering
- Citation
- BIOENGINEERING & TRANSLATIONAL MEDICINE, v.8, no.2
- Journal Title
- BIOENGINEERING & TRANSLATIONAL MEDICINE
- Volume
- 8
- Number
- 2
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87394
- DOI
- 10.1002/btm2.10423
- ISSN
- 2380-6761
- Abstract
- Herein, we report the first study to create a three-dimensional (3D) bioprinted artificial larynx for whole-laryngeal replacement. Our 3D bio-printed larynx was generated using extrusion-based 3D bioprinter with rabbit's chondrocyte-laden gelatin methacryloyl (GelMA)/glycidyl-methacrylated hyaluronic acid (GMHA) hybrid bioink. We used a polycaprolactone (PCL) outer framework incorporated with pores to achieve the structural strength of printed constructs, as well as to provide a suitable microenvironment to support printed cells. Notably, we established a novel fluidics supply (FS) system that simultaneously supplies basal medium together with a 3D bioprinting process, thereby improving cell survival during the printing process. Our results showed that the FS system enhanced post-printing cell viability, which enabled the generation of a large-scale cell-laden artificial laryngeal framework. Additionally, the incorporation of the PCL outer framework with pores and inner hydrogel provides structural stability and sufficient nutrient/oxygen transport. An animal study confirmed that the transplanted 3D bio-larynx successfully maintained the airway. With further development, our new strategy holds great potential for fabricating human-scale larynxes with in vivo-like biological functions for laryngectomy patients.
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