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Electrical pulse stimulation induces differential responses in insulin action in myotubes from severely obese individualsopen access

Authors
Park, SangheeTurner, Kristen D.Zheng, DonghaiBrault, Jeffrey J.Zou, KaiChaves, Alec B.Nielsen, Thomas S.Tanner, Charles J.Treebak, Jonas T.Houmard, Joseph A.
Issue Date
Jan-2019
Publisher
WILEY
Keywords
Contractile Activity; Exercise; Insulin Signaling; Skeletal Muscle
Citation
JOURNAL OF PHYSIOLOGY-LONDON, v.597, no.2, pp.449 - 466
Journal Title
JOURNAL OF PHYSIOLOGY-LONDON
Volume
597
Number
2
Start Page
449
End Page
466
URI
https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87484
DOI
10.1113/JP276990
ISSN
0022-3751
Abstract
Exercise/muscle contraction can enhance whole-body insulin sensitivity; however, in humans the range of improvements can vary substantially. In order, to determine if obesity influences the magnitude of the exercise response, this study compared the effects of electrical pulse stimulation (EPS)-induced contractile activity upon primary myotubes derived from lean and severely obese (BMI >= 40 kg/m(2)) women. Prior to muscle contraction, insulin action was compromised in myotubes from the severely obese as was evident from reduced insulin-stimulated glycogen synthesis, glucose oxidation, glucose uptake, insulin signal transduction (IRS1, Akt, TBC1D4), and insulin-stimulated GLUT4 translocation. EPS (24 h) increased AMP, IMP, AMPK Thr(172) phosphorylation, PGC1 content, and insulin action in myotubes of both the lean and severely obese subjects. However, despite normalizing indices of insulin action to levels seen in the lean control (non-EPS) condition, responses to EPS were blunted with obesity. EPS improved insulin signal transduction in myotubes from lean but not severely obese subjects and EPS increased AMP accumulation and AMPK Thr(172) phosphorylation, but to a lesser degree in myotubes from the severely obese. These data reveal that myotubes of severely obese individuals enhance insulin action and stimulate exercise-responsive molecules with contraction, but in a manner and magnitude that differs from lean subjects.
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