Protective effects of hepatocyte growth factor gene overexpression against hydrogen peroxide-induced apoptosis in mesenchymal stem cells
- Authors
- Choi, Yong Jun; Lee, Chang Min; Lee, Jeong Hyun; Park, See-Hyoung; Nam, Myeong Jin
- Issue Date
- Nov-2019
- Publisher
- WILEY
- Keywords
- CRISPR; hepatocyte growth factor; mesenchymal stem cell; reactive oxygen species
- Citation
- ENVIRONMENTAL TOXICOLOGY, v.34, no.11, pp.1236 - 1245
- Journal Title
- ENVIRONMENTAL TOXICOLOGY
- Volume
- 34
- Number
- 11
- Start Page
- 1236
- End Page
- 1245
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/876
- DOI
- 10.1002/tox.22824
- ISSN
- 1520-4081
- Abstract
- Hepatocyte growth factor (HGF) has recently been reported to exhibit antioxidant and antiapoptotic effects. Therefore, we investigated the effect of overexpression of HGF gene in H2O2-treated mesenchymal stem cells (MSCs). HGF-overexpression increased the cell viability from 50% to 84%, decreased the population of apoptotic cells from 20% to 16%, and decreased the intracellular reactive oxygen species (ROS) levels from 127% to 100% in cells treated with H2O2. HGF suppression decreased the cell viability from 58% to 36%, increased the population of apoptotic cells from 23 to 81%, and increased the intracellular ROS levels from 181% to 240% in cells exposed to H2O2. HGF-overexpression also reduced the expression levels of proapoptotic proteins in MSCs treated with H2O2. Phosphorylation of extracellular signal-regulated kinases, c-Jun amino-terminal kinases, and p38, which was induced by H2O2, decreased in MSCs overexpressing the HGF gene. Taken together, our results suggest that HGF has a protective effect on H2O2-induced apoptosis in MSCs.
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