Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probeopen access
- Authors
- Rajamanikandan, Ramar; Ilanchelian, Malaichamy; Ju, Heongkyu
- Issue Date
- May-2023
- Publisher
- MDPI
- Keywords
- copper nanoclusters; human urine; catalytic oxidation; uric acid detection; uricase; fluorescence
- Citation
- CHEMOSENSORS, v.11, no.5
- Journal Title
- CHEMOSENSORS
- Volume
- 11
- Number
- 5
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88064
- DOI
- 10.3390/chemosensors11050268
- ISSN
- 2227-9040
- Abstract
- We reported on uric acid (UA) detection using a new fluorescence-based assay: poly-(vinylpyrrolidone) templated copper nanoclusters (PVP-CuNCs) with uricase in an aqueous medium, such as human urine with uricase. These nanoclusters were synthesized in a simple wet chemical method and their morphological and optical properties were examined with the aid of high-resolution transmission electron microscopy and optical absorbance/emission spectroscopy. The PVP-CuNCs acted as the fluorescence indicators that used the enzyme-catalyzed oxidation of UA with uricase. Adding UA into the hybrid PVP-CuNCs/uricase solution caused enzyme-catalyzed oxidation to occur, producing hydrogen peroxide (H2O2), allantoin, and carbon dioxide. The fluorescence intensity of PVP-CuNCs is decreased by this biocatalytically generated H2O2, and this decrease is proportional to the UA level. A calibration plot showed the linear relationship with the negative slope between fluorescence intensity and UA in the range of 5-100 x 10(-7) mol/L. The limit of detection (LOD) of UA was estimated as 113 x 10(-9) mol/L. This fluorescent probe turned out to be highly specific for UA over other biologically relevant molecules. The demonstrated capability of the PVP-CuNCs as the nanoprobes for quantification of the UA levels in human urine samples could potentially pave the way toward medical applications where a super-sensitive, cost-effective, and UA-specific diagnosis was required.
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