Human Regulatory Macrophages Derived from THP-1 Cells Using Arginylglycylaspartic Acid and Vitamin D3
DC Field | Value | Language |
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dc.contributor.author | Pham, Hoang Lan | - |
dc.contributor.author | Hoang, Thi Xoan | - |
dc.contributor.author | Kim, Jae Young | - |
dc.date.accessioned | 2023-07-22T00:40:22Z | - |
dc.date.available | 2023-07-22T00:40:22Z | - |
dc.date.created | 2023-07-21 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 2227-9059 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88562 | - |
dc.description.abstract | Regulatory macrophages (Mregs) are unique in that they have anti-inflammatory and immunosuppressive properties. Thus, treating inflammatory diseases using Mregs is an area of active research. Human Mregs are usually generated by culturing peripheral blood monocytes stimulated using a macrophage colony-stimulating factor with interferon (IFN)-& gamma;. Herein, we generated Mregs with an elongated cell morphology from THP-1 cells that were stimulated with phorbol 12-myristate 13-acetate and cultured with both arginylglycylaspartic acid and vitamin D3. These Mregs regulated macrophage function, and respectively downregulated and upregulated the expression of pro-inflammatory and immunosuppressive mediators. They also expressed Mregs-specific markers, such as dehydrogenase/reductase 9, even when exposed to such inflammatory stimulants as IFN-& gamma;, lipopolysaccharide, purified xenogeneic antigen, and xenogeneic cells. The Mregs also exerted anti-inflammatory and anticoagulatory actions in response to xenogeneic cells, as well as exerting immunosuppressive effects on mitogen-induced Jurkat T-cell proliferation. Our method of generating functional Mregs in vitro without cytokines is simple and cost-effective. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | BIOMEDICINES | - |
dc.title | Human Regulatory Macrophages Derived from THP-1 Cells Using Arginylglycylaspartic Acid and Vitamin D3 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 001016883200001 | - |
dc.identifier.doi | 10.3390/biomedicines11061740 | - |
dc.identifier.bibliographicCitation | BIOMEDICINES, v.11, no.6 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85163822528 | - |
dc.citation.title | BIOMEDICINES | - |
dc.citation.volume | 11 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Pham, Hoang Lan | - |
dc.contributor.affiliatedAuthor | Hoang, Thi Xoan | - |
dc.contributor.affiliatedAuthor | Kim, Jae Young | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | regulatory macrophage | - |
dc.subject.keywordAuthor | arginylglycylaspartic acid | - |
dc.subject.keywordAuthor | vitamin D3 | - |
dc.subject.keywordAuthor | anti-inflammation | - |
dc.subject.keywordAuthor | xenotransplantation | - |
dc.subject.keywordPlus | ALTERNATIVE ACTIVATION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | POLARIZATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PHENOTYPE | - |
dc.subject.keywordPlus | TLR10 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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