Diminazene aceturate: An antibacterial agent for Shiga-toxin-producing Escherichia coli O157:H7
DC Field | Value | Language |
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dc.contributor.author | Wu, S.-Y. | - |
dc.contributor.author | Park, G.-Y. | - |
dc.contributor.author | Kim, S.-H. | - |
dc.contributor.author | Hulme, J. | - |
dc.contributor.author | An, S.S.A. | - |
dc.date.available | 2020-02-28T03:44:33Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 1177-8881 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8872 | - |
dc.description.abstract | The aim of this study was to investigate the bacteriostatic and bactericidal effects of diminazene aceturate (DA) against five strains of pathogenic bacteria and two strains of nonpathogenic bacteria. The results showed that 5 µg/mL of DA suppressed the growth of pathogenic Escherichia coli by as much as 77% compared with the controls. Enterohemorrhagic E. coli EDL933 (an E. coli O157:H7 strain) was the most sensitive to DA with a minimum inhibitory concentration of 20 µg/mL. Additional investigations showed that DA induced the highest level of intracellular reactive oxygen species in EDL933. A positive correlation between the reactive oxygen species levels and DA concentration was demonstrated. DA (5 µg/mL) was also a potent uncoupler, inducing a stationary phase collapse (70%–75%) in both strains of E. coli O157:H7. Further investigation showed that the collapse was due to the NaCl:DA ratio in the broth and was potassium ion dependent. A protease screening assay was conducted to elucidate the underlying mechanism. It was found that at neutral pH, the hydrolysis of H-Asp-pNA increased by a factor of 2–3 in the presence of DA, implying that DA causes dysregulation of the proton motive force and a decrease in cellular pH. Finally, a commercial verotoxin test showed that DA did not significantly increase toxin production in EDL933 and was a suitable antibacterial agent for Shiga-toxin-producing E. coli. © 2016 Wu et al. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Dove Medical Press Ltd. | - |
dc.relation.isPartOf | Drug Design, Development and Therapy | - |
dc.title | Diminazene aceturate: An antibacterial agent for Shiga-toxin-producing Escherichia coli O157:H7 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.2147/DDDT.S114832 | - |
dc.identifier.bibliographicCitation | Drug Design, Development and Therapy, v.10, pp.3363 - 3378 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84994048609 | - |
dc.citation.endPage | 3378 | - |
dc.citation.startPage | 3363 | - |
dc.citation.title | Drug Design, Development and Therapy | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Wu, S.-Y. | - |
dc.contributor.affiliatedAuthor | Park, G.-Y. | - |
dc.contributor.affiliatedAuthor | Hulme, J. | - |
dc.contributor.affiliatedAuthor | An, S.S.A. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Berenil© | - |
dc.subject.keywordAuthor | E. coli O157 | - |
dc.subject.keywordAuthor | Enterohemorrhagic | - |
dc.subject.keywordAuthor | Protease | - |
dc.subject.keywordAuthor | Reversible | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordPlus | diminazene aceturate | - |
dc.subject.keywordPlus | potassium ion | - |
dc.subject.keywordPlus | proteinase | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | antiinfective agent | - |
dc.subject.keywordPlus | diminazene | - |
dc.subject.keywordPlus | diminazene aceturate | - |
dc.subject.keywordPlus | peptide hydrolase | - |
dc.subject.keywordPlus | Shiga toxin | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | bacterial cell | - |
dc.subject.keywordPlus | bacterial growth | - |
dc.subject.keywordPlus | bacterial strain | - |
dc.subject.keywordPlus | bactericidal activity | - |
dc.subject.keywordPlus | cell pH | - |
dc.subject.keywordPlus | cell structure | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | Enterococcus faecalis | - |
dc.subject.keywordPlus | enterohemorrhagic Escherichia coli | - |
dc.subject.keywordPlus | enzyme activity | - |
dc.subject.keywordPlus | growth inhibition | - |
dc.subject.keywordPlus | Listeria monocytogenes | - |
dc.subject.keywordPlus | minimum inhibitory concentration | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | Shiga toxin producing Escherichia coli | - |
dc.subject.keywordPlus | Staphylococcus aureus | - |
dc.subject.keywordPlus | analogs and derivatives | - |
dc.subject.keywordPlus | antagonists and inhibitors | - |
dc.subject.keywordPlus | chemistry | - |
dc.subject.keywordPlus | drug effects | - |
dc.subject.keywordPlus | Escherichia coli O157 | - |
dc.subject.keywordPlus | high throughput screening | - |
dc.subject.keywordPlus | Anti-Bacterial Agents | - |
dc.subject.keywordPlus | Diminazene | - |
dc.subject.keywordPlus | Escherichia coli O157 | - |
dc.subject.keywordPlus | High-Throughput Screening Assays | - |
dc.subject.keywordPlus | Peptide Hydrolases | - |
dc.subject.keywordPlus | Shiga Toxin | - |
dc.subject.keywordPlus | Shiga Toxins | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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