A novel drying film culture method applying a natural phenomenon: Increased carotenoid production by<i> Haematococcus</i> sp
- Authors
- Ryu, Yong-Kyun; Lee, Won-Kyu; Choi, Woon-Yong; Kim, Taihun; Lee, Yeon-Ji; Park, Areumi; Kim, Taeho; Oh, Chulhong; Heo, Soo-Jin; Kim, Ji Hyung; Jeon, Ga Eun; Kang, Do-Hyung
- Issue Date
- Dec-2023
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Microalga; Haematococcus; Biomass; Carotenoid; Affordable culture system
- Citation
- BIORESOURCE TECHNOLOGY, v.390
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 390
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89572
- DOI
- 10.1016/j.biortech.2023.129827
- ISSN
- 0960-8524
1873-2976
- Abstract
- Low productivity and high cost remain major bottlenecks for the large-scale production of Haematococcus sp. This study explored biomass production and carotenoid accumulation in Haematococcus sp. (KCTC 12348BP) using drying film culture. The broth-cultured strain (3.2 x 106 cells/mL, 0.83 +/- 0.02 mg/mL for a 21 d culture) was cultured under various conditions (different inoculum volumes and mist feeding intervals) in waterless agar plates at 28 +/- 0.5 degrees C, under fluorescent light (12 h light-dark cycle) for 1 month. The maximum biomass obtained was 17.60 +/- 0.72 g/m2, while the maximum astaxanthin concentration was 8.23 +/- 1.13 mg/g in the culture using 1 mL inoculum and 3 d feeding interval. Drought stress in drying film culture effectively induced the accumulation of carotenoids from beta-carotene, facilitating the production of canthaxanthin via the astaxanthin biosynthesis pathway. This cost-effective culture system can increase the biomass and carotenoid pigment production in Haematococcus sp.
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