Determination of Sufentanil in Human Plasma Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry (UPLC-MS/MS)
DC Field | Value | Language |
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dc.contributor.author | Lee, Sang-Min | - |
dc.contributor.author | Kim, Suin | - |
dc.contributor.author | Shin, Dongseong | - |
dc.contributor.author | Shin, Kwang-Hee | - |
dc.date.accessioned | 2023-12-28T01:30:19Z | - |
dc.date.available | 2023-12-28T01:30:19Z | - |
dc.date.issued | 2023-09 | - |
dc.identifier.issn | 1573-4110 | - |
dc.identifier.issn | 1875-6727 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89836 | - |
dc.description.abstract | Background Sufentanil, an opioid analgesic, is used as an induction agent for general anesthesia during surgery. Sufentanil is more active than other anesthetics and has a narrow therapeutic range. Therefore, a precise dosage regimen is necessary when administering sufentanil.Objective This study aimed to develop a bioanalytical method for the determination of sufentanil within the sensitive concentration range more than that in a previous study utilizing protein precipitation (PP) and determine the plasma concentration of sufentanil.Methods A method for quantitating sufentanil was developed using ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) and detection by electrospray ionization (ESI). The internal standard was sufentanil-d5. Chromatographic separation was performed using an Acquity UPLC HSS T3 column (50 x 2.1 mm, 1.8 mu m) from Waters (Milford, MA, USA). Protein precipitation (PP) was used for sample preparation, and gradient elution was conducted using a mobile phase consisting of 1 mL of 2M ammonium acetate in 99% formic acid in 1 L of water or in 1 L of acetonitrile. The total run time for the analysis was 5 min, and the flow rate was 0.4 mL/min.Results Standard curves were linear over ranges of 0.025-30 ng/mL for sufentanil with a correlation coefficient (r2) greater than 0.9998. The lower limit of quantification (LLOQ) was 0.025 ng/mL. The intra- and inter-day accuracies were 97.66%-108.8% and 101.25%-103.17%, respectively, and the precision did not exceed 15% for sufentanil.Conclusion This study developed a validated, simple, sensitive, and convenient UPLC-MS/MS method for quantifying sufentanil in human plasma. This method was applied to determine sufentanil concentration in the plasma of patients undergoing surgery. The bioanalytical method using PP showed a sufficiently sensitive concentration range. This method could be applied to various pharmacokinetic studies of sufentanil. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BENTHAM SCIENCE PUBL LTD | - |
dc.title | Determination of Sufentanil in Human Plasma Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry (UPLC-MS/MS) | - |
dc.type | Article | - |
dc.identifier.wosid | 001117125900005 | - |
dc.identifier.doi | 10.2174/1573411019666230823094749 | - |
dc.identifier.bibliographicCitation | CURRENT ANALYTICAL CHEMISTRY, v.19, no.7, pp 531 - 540 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85180221603 | - |
dc.citation.endPage | 540 | - |
dc.citation.startPage | 531 | - |
dc.citation.title | CURRENT ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 7 | - |
dc.type.docType | Article | - |
dc.publisher.location | 아랍에미리트 | - |
dc.subject.keywordAuthor | Sufentanil | - |
dc.subject.keywordAuthor | human plasma | - |
dc.subject.keywordAuthor | bioanalysis | - |
dc.subject.keywordAuthor | UPLC-MS/MS | - |
dc.subject.keywordAuthor | protein precipitation (PP) | - |
dc.subject.keywordAuthor | MRM | - |
dc.subject.keywordPlus | HUMAN SERUM | - |
dc.subject.keywordPlus | PHARMACOKINETICS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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