Determination of Sufentanil in Human Plasma Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry (UPLC-MS/MS)
- Authors
- Lee, Sang-Min; Kim, Suin; Shin, Dongseong; Shin, Kwang-Hee
- Issue Date
- Sep-2023
- Publisher
- BENTHAM SCIENCE PUBL LTD
- Keywords
- Sufentanil; human plasma; bioanalysis; UPLC-MS/MS; protein precipitation (PP); MRM
- Citation
- CURRENT ANALYTICAL CHEMISTRY, v.19, no.7, pp 531 - 540
- Pages
- 10
- Journal Title
- CURRENT ANALYTICAL CHEMISTRY
- Volume
- 19
- Number
- 7
- Start Page
- 531
- End Page
- 540
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89836
- DOI
- 10.2174/1573411019666230823094749
- ISSN
- 1573-4110
1875-6727
- Abstract
- Background Sufentanil, an opioid analgesic, is used as an induction agent for general anesthesia during surgery. Sufentanil is more active than other anesthetics and has a narrow therapeutic range. Therefore, a precise dosage regimen is necessary when administering sufentanil.Objective This study aimed to develop a bioanalytical method for the determination of sufentanil within the sensitive concentration range more than that in a previous study utilizing protein precipitation (PP) and determine the plasma concentration of sufentanil.Methods A method for quantitating sufentanil was developed using ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) and detection by electrospray ionization (ESI). The internal standard was sufentanil-d5. Chromatographic separation was performed using an Acquity UPLC HSS T3 column (50 x 2.1 mm, 1.8 mu m) from Waters (Milford, MA, USA). Protein precipitation (PP) was used for sample preparation, and gradient elution was conducted using a mobile phase consisting of 1 mL of 2M ammonium acetate in 99% formic acid in 1 L of water or in 1 L of acetonitrile. The total run time for the analysis was 5 min, and the flow rate was 0.4 mL/min.Results Standard curves were linear over ranges of 0.025-30 ng/mL for sufentanil with a correlation coefficient (r2) greater than 0.9998. The lower limit of quantification (LLOQ) was 0.025 ng/mL. The intra- and inter-day accuracies were 97.66%-108.8% and 101.25%-103.17%, respectively, and the precision did not exceed 15% for sufentanil.Conclusion This study developed a validated, simple, sensitive, and convenient UPLC-MS/MS method for quantifying sufentanil in human plasma. This method was applied to determine sufentanil concentration in the plasma of patients undergoing surgery. The bioanalytical method using PP showed a sufficiently sensitive concentration range. This method could be applied to various pharmacokinetic studies of sufentanil.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.