Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilmsopen access
- Authors
- Kim, Hee-Eun
- Issue Date
- Feb-2024
- Publisher
- MDPI
- Keywords
- antibacterial effect; cold atmospheric plasma; microcosm biofilm; oral biofilm; quantitative light-induced fluorescence-digital
- Citation
- Applied Sciences-basel, v.14, no.3
- Journal Title
- Applied Sciences-basel
- Volume
- 14
- Number
- 3
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/90276
- DOI
- 10.3390/app14031211
- ISSN
- 2076-3417
2076-3417
- Abstract
- This study aimed to compare the longitudinal efficacy between chlorhexidine gluconate (CHX; 0.12%) and cold atmospheric plasma (CAP) in reducing oral biofilm pathogenicity, utilizing a quantitative light-induced fluorescence-digital (QLF-D) camera. Oral microcosm biofilms were developed for 2 days on 57 hydroxyapatite disks. These biofilms were treated with distilled water for 1 min, CHX for 1 min, and CAP for 2 min over the course of 6 days. The red fluorescence intensities of the biofilms were measured using a QLF-D and expressed as pre- and post-treatment red/green ratios (Ratio(R/G)). The bacterial viability (ratio of the green-stained area to the total stained area, Ratio(G/G+R)) was calculated using live/dead bacterial staining; the total and aciduric bacterial counts were determined. A significant intergroup difference was found between Ratio(R/G) changes according to the treatment period (p < 0.001). The Ratio(R/G) observed within the CAP-treated group was significantly lower compared with the CHX-treated group at every interval of measurement (p < 0.001). The CAP-treated group also exhibited a lower Ratio(G/G+R) and more weakened bacterial aggregation compared with the CHX-treated group (p < 0.05). In the group treated with CAP, the counts of both total and aciduric bacteria were substantially reduced compared with the DW group, with a statistically significant reduction (p < 0.001). Therefore, CAP may be more effective in minimizing oral microcosm biofilm pathogenicity than 0.12% CHX.
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