Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in children
DC Field | Value | Language |
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dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Park, Pil-gu | - |
dc.contributor.author | Hwang, Su Jin | - |
dc.contributor.author | Bang, Seung Jun | - |
dc.contributor.author | Jung, Jae Hwa | - |
dc.contributor.author | Kown, Eun Bin | - |
dc.contributor.author | Sul, Eun Kyung | - |
dc.contributor.author | Song, Kyung Chul | - |
dc.contributor.author | Choi, Joon-sik | - |
dc.contributor.author | Han, Kyeo Re | - |
dc.contributor.author | Lee, Hye Young | - |
dc.contributor.author | Kim, Dong Hyun | - |
dc.contributor.author | Lee, Jae Myun | - |
dc.date.accessioned | 2024-04-12T00:00:47Z | - |
dc.date.available | 2024-04-12T00:00:47Z | - |
dc.date.issued | 2024-01 | - |
dc.identifier.issn | 0146-6615 | - |
dc.identifier.issn | 1096-9071 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/90960 | - |
dc.description.abstract | Early and accurate detection of viruses in children might help prevent transmission and severe diseases. In this study, the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) detection in children was evaluated using saliva specimens with a Proteinase K (PTK)-based RNA preparation, as saliva collection is a simple and noninvasive procedure, even in young children, with fewer concerns about sample contamination. The saliva-based PTK and the conventional paired nasopharyngeal aspiration (NPA)-based detection methods were compared between COVID-19-positive and -negative children. In addition, the detection rate for SARS-COV-2 and the difference between admission and discharge by the saliva-based PTK method was tested in COVID-19 patients. The diagnostic accuracy of the saliva-based PTK method was 98.8% compared to NP swab-based reverse transcriptase polymerase chain reaction. Saliva samples showed high sensitivity (94.1%) and specificity (100%) when using the PTK method. Furthermore, the saliva-based PTK method significantly reduced the test processing time by 2 h. Notably, Ct values at discharge increased in saliva samples compared with those at admission, which might indicate patients' clinical conditions or virus activity. In conclusion, the saliva-based PTK implemented in this study streamlines RNA extraction, making the process faster, safer, and more cost-effective, demonstrating that this method is a rapid and reliable diagnostic tool for SARS-CoV-2 detection in children. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in children | - |
dc.type | Article | - |
dc.identifier.wosid | 001158783800039 | - |
dc.identifier.doi | 10.1002/jmv.29361 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MEDICAL VIROLOGY, v.96, no.1 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85181630116 | - |
dc.citation.title | JOURNAL OF MEDICAL VIROLOGY | - |
dc.citation.volume | 96 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | children | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | diagnostics | - |
dc.subject.keywordAuthor | Proteinase K | - |
dc.subject.keywordAuthor | saliva | - |
dc.subject.keywordAuthor | SARS-COV-2 | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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