Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in childrenopen access
- Authors
- Kim, Min Jung; Park, Pil-gu; Hwang, Su Jin; Bang, Seung Jun; Jung, Jae Hwa; Kown, Eun Bin; Sul, Eun Kyung; Song, Kyung Chul; Choi, Joon-sik; Han, Kyeo Re; Lee, Hye Young; Kim, Dong Hyun; Lee, Jae Myun
- Issue Date
- Jan-2024
- Publisher
- WILEY
- Keywords
- children; COVID-19; diagnostics; Proteinase K; saliva; SARS-COV-2
- Citation
- JOURNAL OF MEDICAL VIROLOGY, v.96, no.1
- Journal Title
- JOURNAL OF MEDICAL VIROLOGY
- Volume
- 96
- Number
- 1
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/90960
- DOI
- 10.1002/jmv.29361
- ISSN
- 0146-6615
1096-9071
- Abstract
- Early and accurate detection of viruses in children might help prevent transmission and severe diseases. In this study, the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) detection in children was evaluated using saliva specimens with a Proteinase K (PTK)-based RNA preparation, as saliva collection is a simple and noninvasive procedure, even in young children, with fewer concerns about sample contamination. The saliva-based PTK and the conventional paired nasopharyngeal aspiration (NPA)-based detection methods were compared between COVID-19-positive and -negative children. In addition, the detection rate for SARS-COV-2 and the difference between admission and discharge by the saliva-based PTK method was tested in COVID-19 patients. The diagnostic accuracy of the saliva-based PTK method was 98.8% compared to NP swab-based reverse transcriptase polymerase chain reaction. Saliva samples showed high sensitivity (94.1%) and specificity (100%) when using the PTK method. Furthermore, the saliva-based PTK method significantly reduced the test processing time by 2 h. Notably, Ct values at discharge increased in saliva samples compared with those at admission, which might indicate patients' clinical conditions or virus activity. In conclusion, the saliva-based PTK implemented in this study streamlines RNA extraction, making the process faster, safer, and more cost-effective, demonstrating that this method is a rapid and reliable diagnostic tool for SARS-CoV-2 detection in children.
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