Heat-Treated Paraprobiotic Latilactobacillus sakei KU15041 and Latilactobacillus<i> curvatus</i> KU15003 Show an Antioxidant and Immunostimulatory Effectopen access
- Authors
- Hyun, Jun-Hyun; Woo, Im-Kyung; Kim, Kee-Tae; Park, Young-Seo; Kang, Dae-Kyung; Lee, Na-Kyoung; Paik, Hyun-Dong
- Issue Date
- Feb-2024
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Antioxidant effect; immunostimulatory effect; paraprobiotics; Latilactobacillus sakei; Latilactobacillus curvatus
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.34, no.2, pp 358 - 366
- Pages
- 9
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 34
- Number
- 2
- Start Page
- 358
- End Page
- 366
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/91404
- DOI
- 10.4014/jmb.2309.09007
- ISSN
- 1017-7825
1738-8872
- Abstract
- The lactic acid bacteria, including Latilactobacillus sakei and Latilactobacillus curvatus, have been widely studied for their preventive and therapeutic effects. In this study, the underlying mechanism of action for the antioxidant and immunostimulatory effects of two strains of heat -treated paraprobiotics was examined. Heat -treated L. sakei KU15041 and L. curvatus KU15003 showed higher radical scavenging activity in both the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays than the commercial probiotic strain LGG. In addition, treatment with these two strains exhibited immunostimulatory effects in RAW 264.7 macrophages, with L. curvatus KU15003 showing a slightly higher effect. Additionally, they promoted phagocytosis and NO production in RAW 264.7 cells without any cytotoxicity. Moreover, the expression of tumor necrosis factor-alpha, interleukin (IL)-1 beta, and IL -6 was upregulated. These strains resulted in an increased expression of inducible nitric oxide synthase and cyclooxygenase-2. Moreover, the nuclear factor -KB and mitogen-activated protein kinase signaling pathways were stimulated by these strains. These findings suggest the potential of using L. sakei KU15041 and L. curvatus KU15003 in food or by themselves as probiotics with antioxidant and immune -enhancing properties.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/91404)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.