The branching ratio of enzymatically synthesized α-glucans impacts microbiome and metabolic outcomes of <i>in vitro </i>fecal fermentation
DC Field | Value | Language |
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dc.contributor.author | Yasar, Arife | - |
dc.contributor.author | Ryu, Hye-Jung | - |
dc.contributor.author | Esen, Emine | - |
dc.contributor.author | Sarioglan, Ihsan | - |
dc.contributor.author | Deemer, Dane | - |
dc.contributor.author | Cetin, Buelent | - |
dc.contributor.author | Yoo, Sang-Ho | - |
dc.contributor.author | Lindemann, Stephen R. | - |
dc.contributor.author | Lee, Byung-Hoo | - |
dc.contributor.author | Tuncil, Yunus E. | - |
dc.date.accessioned | 2024-06-19T13:00:21Z | - |
dc.date.available | 2024-06-19T13:00:21Z | - |
dc.date.issued | 2024-07 | - |
dc.identifier.issn | 0144-8617 | - |
dc.identifier.issn | 1879-1344 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/91564 | - |
dc.description.abstract | The aim of this study was to evaluate the impacts of enzymatically synthesized alpha-glucans possessing alpha-1,4- and alpha-1,6-glucose linkages, and varying in branching ratio, on colonic microbiota composition and metabolic function. Four different alpha-glucans varying in branching ratio were synthesized by amylosucrase from Neisseria polysaccharea and glycogen branching enzyme from Rhodothermus obamensis. The branching ratios were found to range from 0 % to 2.8 % using GC/MS. In vitro fecal fermentation analyses (n = 8) revealed that the branching ratio dictates the short-chain fatty acid (SCFA) generation by fecal microbiota. Specifically, slightly branched (0.49 %) alpha-glucan resulted in generation of significantly (P < 0.05) higher amounts of propionate, compared to more-branched counterparts. In addition, the amount of butyrate generated from this alpha-glucan was statistically (P > 0.05) indistinguishable than those observed in resistant starches. 16S rRNA sequencing revealed that enzymatically synthesized alpha-glucans stimulated Lachnospiraceae and Ruminococcus related OTUs. Overall, the results demonstrated metabolic function of colonic microbiota can be manipulated by altering the branching ratio of enzymatically synthesized alpha-glucans, providing insights into specific structure-function relationships between dietary fibers and the colonic microbiome. Furthermore, the slightly branched alpha-glucans could be used as functional carbohydrates to stimulate the beneficial microbiota and SCFAs in the colon. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | The branching ratio of enzymatically synthesized α-glucans impacts microbiome and metabolic outcomes of <i>in vitro </i>fecal fermentation | - |
dc.type | Article | - |
dc.identifier.wosid | 001221637600001 | - |
dc.identifier.doi | 10.1016/j.carbpol.2024.122087 | - |
dc.identifier.bibliographicCitation | CARBOHYDRATE POLYMERS, v.335 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85189003198 | - |
dc.citation.title | CARBOHYDRATE POLYMERS | - |
dc.citation.volume | 335 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Colon | - |
dc.subject.keywordAuthor | Microbiome | - |
dc.subject.keywordAuthor | 16S rRNA | - |
dc.subject.keywordAuthor | Glucan | - |
dc.subject.keywordAuthor | Amylosucrase | - |
dc.subject.keywordAuthor | Glycogen branching enzyme | - |
dc.subject.keywordPlus | DIETARY FIBER STRUCTURES | - |
dc.subject.keywordPlus | RESISTANT STARCH | - |
dc.subject.keywordPlus | GUT | - |
dc.subject.keywordPlus | ARABINOXYLAN | - |
dc.subject.keywordPlus | COLON | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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