Electrospun fibers modified with polydopamine for enhancing human mesenchymal stem cell culture
- Authors
- Yang, Da Hyun; Lee, Nae Yoon
- Issue Date
- Jul-2024
- Publisher
- IOP Publishing Ltd
- Keywords
- polydopamine; RGD; mesenchymal stem cell; electrospinning; fibrous scaffolds
- Citation
- BIOMEDICAL MATERIALS, v.19, no.4
- Journal Title
- BIOMEDICAL MATERIALS
- Volume
- 19
- Number
- 4
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/91671
- DOI
- 10.1088/1748-605X/ad49f7
- ISSN
- 1748-6041
1748-605X
- Abstract
- In this study, we coated electrospun polycaprolactone (PCL) fibers with polydopamine (PDA) to modify their hydrophobicity and fabricated a matrix for culturing mesenchymal stem cells (MSCs). Additionally, we incorporated Arg-Gly-Asp (RGD) peptides into PDA to enhance MSCs culture performance on PCL fibers. PDA and RGD were successfully coated in one step by immersing the electrospun fibers in a coating solution, without requiring an additional surface activation process. The characteristics of functionalized PCL fibers were analyzed by scanning electron microscopy with energy-dispersive x-ray analysis, Fourier transform infrared spectroscopy, water contact angle measurement, and fluorescence measurements using a carboxylic-modified fluorescent microsphere. MSCs cultured on the modified PCL fibers demonstrated enhanced cell adhesion, proliferation, and osteogenic- and chondrogenic differentiation. This study provides insight into potential applications for scaffold fabrication in MSCs-based tissue engineering, wound dressing, implantation, and a deeper understanding of MSCs behavior in vitro.
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