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Label-Free Detection of DNA Polymerase Activity Using Hairpin DNA-Templated Silver Nanoclusters
- Authors
- Kim, Seyeon; Gang, Jongback
- Issue Date
- Oct-2019
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Klenow Fragment; DNA Polymerization; Daidzein; DNA-Templated Silver Nanoclusters (DNA-AgNCs)
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.19, no.10, pp.6534 - 6538
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 19
- Number
- 10
- Start Page
- 6534
- End Page
- 6538
- ISSN
- 1533-4880
- Abstract
- Based on the characteristic properties like strong fluorescence and high photostability, DNA-templated silver nanoclusters (DNA-AgNCs) were utilized to detect Klenow fragment exo- (KF-) activity and to monitor its inhibition by daidzein. In this study, fluorescence intensity is linearly correlated for KF-concentration ranging from 0 to 2.0 U/mL, with a minimum detectable concentration of 0.05 U/mL. In addition, KF- inhibition was monitored by changes in fluorescence intensity with increasing daidzein concentration gradually.
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Related Researcher
- Gang, Jong Back
- BioNano Technology (Department of Chemistry)