仙鶴草추출물의 대식세포에서의 LPS-유도 염증반응에 대한 효능 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황지혜 | - |
dc.contributor.author | 남주현 | - |
dc.contributor.author | 김우경 | - |
dc.contributor.author | 배효상 | - |
dc.date.available | 2020-02-28T06:41:22Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 1229-1765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9548 | - |
dc.description.abstract | Objectives : The aerial parts of Agrimonia pilosa Ledeb (Agrimoniae Herba; AH) has been traditionally used as a Korean medicine to treatment of abdominal pain, sore throat, headaches, bloody discharge, parasitic infections and eczema. In this study, we investigated the effect of AH ethanol extract on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophage cells. Methods : AH was extracted by 30% ethanol (AH-E). Raw264.7 cells were treated with AH-E extract at different concentrations for 30 min and then stimulated with LPS (1㎍/㎖) or without for indicated times. Cell viability was measured by MTT assay, and nitric oxide (NO) production was measured by Griess assay. The expression of inflammatory mediators, iNOS and COX-2 and inflammatory cytokines, TNF-α, IL-1β, and IL-6 was detected by RT-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was analyzed by Western blot. Also, the expression of NF-κB in nuclear and cytosol was detected by Western blot. Results : AH-E extract significantly decreased LPS-induced NO production in RAW264.7 cells. AH-E extract inhibited the mRNA expression of iNOS, COX-2, TNF-α, IL-1β, and IL-6 in LPS-stimulated cells with a dose-dependent manner. In addition, the phosphorylation of ERK, p38 and JNK MAPKs was also inhibited by AH-E extract. AP-E extract inhibited the nuclear translocation of NF-κB in LPS-stimulated cells. Conclusions : Our results suggest that AH-E extract has an anti-inflammatory activity in macrophages-mediated inflammation. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한본초학회 | - |
dc.relation.isPartOf | 대한본초학회지 | - |
dc.title | 仙鶴草추출물의 대식세포에서의 LPS-유도 염증반응에 대한 효능 연구 | - |
dc.title.alternative | Effects of Agrimoniae Herba 30% ethanol extract on LPS-induced inflammatory responses in RAW264.7 macrophge cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 대한본초학회지, v.31, no.2, pp.63 - 69 | - |
dc.identifier.kciid | ART002097883 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 69 | - |
dc.citation.startPage | 63 | - |
dc.citation.title | 대한본초학회지 | - |
dc.citation.volume | 31 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | 황지혜 | - |
dc.subject.keywordAuthor | Agrimonia pilosa Ledeb | - |
dc.subject.keywordAuthor | 30% ethanol extract | - |
dc.subject.keywordAuthor | anti-inflammation | - |
dc.subject.keywordAuthor | RAW264.7 cells | - |
dc.description.journalRegisteredClass | kci | - |
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