仙鶴草추출물의 대식세포에서의 LPS-유도 염증반응에 대한 효능 연구Effects of Agrimoniae Herba 30% ethanol extract on LPS-induced inflammatory responses in RAW264.7 macrophge cells
- Other Titles
- Effects of Agrimoniae Herba 30% ethanol extract on LPS-induced inflammatory responses in RAW264.7 macrophge cells
- Authors
- 황지혜; 남주현; 김우경; 배효상
- Issue Date
- Mar-2016
- Publisher
- 대한본초학회
- Keywords
- Agrimonia pilosa Ledeb; 30% ethanol extract; anti-inflammation; RAW264.7 cells
- Citation
- 대한본초학회지, v.31, no.2, pp.63 - 69
- Journal Title
- 대한본초학회지
- Volume
- 31
- Number
- 2
- Start Page
- 63
- End Page
- 69
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9548
- ISSN
- 1229-1765
- Abstract
- Objectives : The aerial parts of Agrimonia pilosa Ledeb (Agrimoniae Herba; AH) has been traditionally used as a Korean medicine to treatment of abdominal pain, sore throat, headaches, bloody discharge, parasitic infections and eczema. In this study, we investigated the effect of AH ethanol extract on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophage cells.
Methods : AH was extracted by 30% ethanol (AH-E). Raw264.7 cells were treated with AH-E extract at different concentrations for 30 min and then stimulated with LPS (1㎍/㎖) or without for indicated times. Cell viability was measured by MTT assay, and nitric oxide (NO) production was measured by Griess assay. The expression of inflammatory mediators, iNOS and COX-2 and inflammatory cytokines, TNF-α, IL-1β, and IL-6 was detected by RT-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was analyzed by Western blot. Also, the expression of NF-κB in nuclear and cytosol was detected by Western blot.
Results : AH-E extract significantly decreased LPS-induced NO production in RAW264.7 cells. AH-E extract inhibited the mRNA expression of iNOS, COX-2, TNF-α, IL-1β, and IL-6 in LPS-stimulated cells with a dose-dependent manner. In addition, the phosphorylation of ERK, p38 and JNK MAPKs was also inhibited by AH-E extract. AP-E extract inhibited the nuclear translocation of NF-κB in LPS-stimulated cells.
Conclusions : Our results suggest that AH-E extract has an anti-inflammatory activity in macrophages-mediated inflammation.
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