Governing effect of regulatory proteins for Cl-/HCO3- exchanger 2 activity
DC Field | Value | Language |
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dc.contributor.author | Jeong, Yon Soo | - |
dc.contributor.author | Hong, Jeong Hee | - |
dc.date.available | 2020-02-28T06:43:41Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1933-6950 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9717 | - |
dc.description.abstract | Anion exchanger 2 (AE2) has a critical role in epithelial cells and is involved in the ionic homeostasis such as Cl- uptake and HCO3- secretion. However, little is known about the regulatory mechanism of AE2. The main goal of the present study was to investigate potential regulators, such as spinophilin (SPL), inositol-1,4,5-trisphosphate [IP3] receptors binding protein released with IP3 (IRBIT), STE20/SPS1-related proline/alanine-rich kinase (SPAK) kinase, and carbonic anhydrase XII (CA XII). We found that SPL binds to AE2 and markedly increased the Cl-/HCO3- exchange activity of AE2. Especially SPL 1-480 domain is required for enhancing AE2 activity. For other regulatory components that affect the fidelity of fluid and HCO3- secretion, IRBIT and SPAK had no effect on the activity of AE2 and no protein-protein interaction with AE2. It has been proposed that CA activity is closely associated with AE activity. In this study, we provide evidence that the basolateral membrane-associated CA isoform CA XII significantly increased the activity of AE2 and co-localized with AE2 to the plasma membrane. Collectively, SPL and CA XII enhanced the Cl-/HCO3- exchange activity of AE2. The modulating action of these regulatory proteins could serve as potential therapeutic targets for secretory diseases mediated by AE2. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.relation.isPartOf | CHANNELS | - |
dc.subject | BINDING-PROTEIN | - |
dc.subject | ACINAR-CELLS | - |
dc.subject | SPINOPHILIN | - |
dc.subject | SECRETION | - |
dc.subject | IRBIT | - |
dc.subject | FLUID | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | TRANSPORTERS | - |
dc.subject | RECEPTORS | - |
dc.subject | PARTNERS | - |
dc.title | Governing effect of regulatory proteins for Cl-/HCO3- exchanger 2 activity | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000374581700007 | - |
dc.identifier.doi | 10.1080/19336950.2015.1134068 | - |
dc.identifier.bibliographicCitation | CHANNELS, v.10, no.3, pp.214 - 224 | - |
dc.identifier.scopusid | 2-s2.0-84978420864 | - |
dc.citation.endPage | 224 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | CHANNELS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Jeong, Yon Soo | - |
dc.contributor.affiliatedAuthor | Hong, Jeong Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | anion exchanger 2 | - |
dc.subject.keywordAuthor | carbonic anhydrase XII | - |
dc.subject.keywordAuthor | fluid secretion | - |
dc.subject.keywordAuthor | IRBIT | - |
dc.subject.keywordAuthor | SPAK | - |
dc.subject.keywordAuthor | spinophilin | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | ACINAR-CELLS | - |
dc.subject.keywordPlus | SPINOPHILIN | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | IRBIT | - |
dc.subject.keywordPlus | FLUID | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | TRANSPORTERS | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | PARTNERS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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