Governing effect of regulatory proteins for Cl-/HCO3- exchanger 2 activity
- Authors
- Jeong, Yon Soo; Hong, Jeong Hee
- Issue Date
- 2016
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- anion exchanger 2; carbonic anhydrase XII; fluid secretion; IRBIT; SPAK; spinophilin
- Citation
- CHANNELS, v.10, no.3, pp.214 - 224
- Journal Title
- CHANNELS
- Volume
- 10
- Number
- 3
- Start Page
- 214
- End Page
- 224
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9717
- DOI
- 10.1080/19336950.2015.1134068
- ISSN
- 1933-6950
- Abstract
- Anion exchanger 2 (AE2) has a critical role in epithelial cells and is involved in the ionic homeostasis such as Cl- uptake and HCO3- secretion. However, little is known about the regulatory mechanism of AE2. The main goal of the present study was to investigate potential regulators, such as spinophilin (SPL), inositol-1,4,5-trisphosphate [IP3] receptors binding protein released with IP3 (IRBIT), STE20/SPS1-related proline/alanine-rich kinase (SPAK) kinase, and carbonic anhydrase XII (CA XII). We found that SPL binds to AE2 and markedly increased the Cl-/HCO3- exchange activity of AE2. Especially SPL 1-480 domain is required for enhancing AE2 activity. For other regulatory components that affect the fidelity of fluid and HCO3- secretion, IRBIT and SPAK had no effect on the activity of AE2 and no protein-protein interaction with AE2. It has been proposed that CA activity is closely associated with AE activity. In this study, we provide evidence that the basolateral membrane-associated CA isoform CA XII significantly increased the activity of AE2 and co-localized with AE2 to the plasma membrane. Collectively, SPL and CA XII enhanced the Cl-/HCO3- exchange activity of AE2. The modulating action of these regulatory proteins could serve as potential therapeutic targets for secretory diseases mediated by AE2.
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