Comparative Analysis of Dibutyric cAMP and Butyric Acid on the Differentiation of Human Eosinophilic Leukemia EoL-1 Cells
- Authors
- Jung, YunJae
- Issue Date
- Dec-2015
- Publisher
- KOREA ASSOC IMMUNOLOGISTS
- Keywords
- EoL-1 cells; DbcAMP; Butyric acid; In vitro differentiation; Eosinophils
- Citation
- IMMUNE NETWORK, v.15, no.6, pp.313 - 318
- Journal Title
- IMMUNE NETWORK
- Volume
- 15
- Number
- 6
- Start Page
- 313
- End Page
- 318
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9863
- DOI
- 10.4110/in.2015.15.6.313
- ISSN
- 1598-2629
- Abstract
- Purification of enough numbers of circulating eosinophils is difficult because eosinophils account for less than 5% peripheral blood leukocytes. Human eosinophilic leukemia EoL-1 cells have been considered an in vitro source of eosinophils as they can differentiate into mature eosinophil-like cells when incubated with dibutyryl cAMP (dbcAMP) or butyric acid. In this study, the viability and phenotypic maturation of EoL-1 cells stimulated by either dbcAMP or butyric acid were comparatively analyzed. After treatment with 100 mu M dbcAMP or 0.5 mu M butyric acid, EoL-1 cells showed morphological signs of differentiation, although the number of nonviable EoL-1 cells was significantly increased following butyric acid treatment. Stimulation of EoL-1 cells with 0.5 mu M butyric acid more effectively induced the expression of mature eosinophil markers than stimulation with dbcAMP. These results suggest that treatment of EoL-1 cells with 0.5 mu M butyric acid for limited duration could be an effective strategy for inducing their differentiation. Considering that expression of CCR3 was not sufficient in EoL-1 cells stimulated with 0.5 mu M butyric acid, treatment of the chemically stimulated EoL-1 cells with cytokines, which primarily support eosinophil maturation, would help to obtain differentiated EoL-1 cells with greater functional maturity.
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