A simple sensing of hazardous photo-induced superoxide anion radicals using a molecular probe in ZnO-Nanoparticles aqueous medium
- Authors
- Agbe, Henry; Raza, Nadeem; Dodoo-Arhin, David; Kumar, R. Vasant; Kim, Ki-Hyun
- Issue Date
- Sep-2019
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Photocatalysis; XTT tetrazolium salt; ZnO-NPs; Molecular probe; Reactive oxygen species; Superoxide anion radicals
- Citation
- ENVIRONMENTAL RESEARCH, v.176, pp.1 - 8
- Indexed
- SCIE
SCOPUS
- Journal Title
- ENVIRONMENTAL RESEARCH
- Volume
- 176
- Start Page
- 1
- End Page
- 8
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/12566
- DOI
- 10.1016/j.envres.2019.03.062
- ISSN
- 0013-9351
- Abstract
- The generation of reactive oxygen species (ROS) during the photolysis of sunscreens and sun blockers poses consumer safety concerns while necessitating proper identification and quantitation of ROS species. Here, a colorimetric sensing approach has been developed based on a molecular probe (2,3-bis-(2-methoxy-4-nitro-5sulfopheny1)-2-H-tetrazolium-5-carboxanilide (XTT) tetrazolium salt) to quantitatively measure the photo-induced superoxide anion radicals (center dot O-2(center dot)) generated from the photocatalysis of zinc oxide nanoparticles (ZnO-NPs) in aqueous solutions. Note that superoxide anion radicals are assumed to be the main reactive oxygen species (ROS) generated from such photocatalysis. The characterisation of ZnO-NPs before and after irradiation showed average particle sizes of 616.5 and 295.3 nm and zeta-potential values of 0 and -24.4 mV, respectively. It is hoped that this proposed protocol can be further developed to efficiently detect other ROS present in inorganic sun blockers and to optimize the utility of various sunscreen formulations.
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