Expansion of the prime editing modality with Cas9 from Francisella novicidaopen access
- Authors
- Oh, Yeounsun; Lee, Wi-jae; Hur, Junho K.; Song, Woo Jeung; Lee, Youngjeon; Kim, Hanseop; Gwon, Lee Wha; Kim, Young-Hyun; Park, Young-Ho; Kim, Chan Hyoung; Lim, Kyung-Seob; Song, Bong-Seok; Huh, Jae-Won; Kim, Sun-Uk; Jun, Bong-Hyun; Jung, Cheulhee; Lee, Seung Hwan
- Issue Date
- Apr-2022
- Publisher
- BMC
- Keywords
- Prime editing; Target expansion; CRISPR-Cas9; Ortholog; Francisella novicida
- Citation
- GENOME BIOLOGY, v.23, no.1, pp.1 - 12
- Indexed
- SCIE
SCOPUS
- Journal Title
- GENOME BIOLOGY
- Volume
- 23
- Number
- 1
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/138910
- DOI
- 10.1186/s13059-022-02644-8
- ISSN
- 1474-760X
- Abstract
- Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.
- Files in This Item
-
- Appears in
Collections - 서울 의과대학 > 서울 유전학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/138910)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.