Efficacy of Human Adipose-Derived Stem Cells in Spinal Fusion in a Rat Ovariectomy Osteoporosis Model
- Authors
- Na, Min Kyun; Bae, In-Suk; Yi, Hyeong-Joong; Do, Byung-Rok; Kim, Kee D.; Chun, Hyoung-Joon
- Issue Date
- Mar-2022
- Publisher
- TURKISH NEUROSURGICAL SOC
- Keywords
- Adipose-derived stem cell; Spinal fusion; Osteoporosis; Ovariectomy
- Citation
- TURKISH NEUROSURGERY, v.32, no.2, pp.251 - 260
- Indexed
- SCIE
SCOPUS
- Journal Title
- TURKISH NEUROSURGERY
- Volume
- 32
- Number
- 2
- Start Page
- 251
- End Page
- 260
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/139291
- DOI
- 10.5137/1019-5149.JTN.33739-21.4
- ISSN
- 1019-5149
- Abstract
- AIM: To evaluate the efficacy of human adipose-derived stem cells (h-ADSCs) in spinal fusion in an osteoporotic rat model.
MATERIAL and METHODS: Female Sprague-Dawley rats (n=40) underwent ovariectomy and were then randomly assigned into two groups: ovariectomy (OVX) (OVX + fusion) and h-ADSCs (OVX + fusion + h-ADSCs). Six weeks after OVX, we performed bilateral lumbar spinal fusion using the autologous iliac bone with or without administration of h-ADSCs. The efficacy of the spinal fusion was then assessed using manual palpation, lateral ending, morphogenic examinations, and histology six weeks the after fusion procedure.
RESULTS: Fusion bed volume was different between the two groups but not significantly. However, the fusion bed density was higher in the h-ADSC group than in the OVX group. Manual palpation (70% vs. 40%, p=0.112) and lateral bending (95% vs. 55%, p=0.011) produced higher fusion rates in the h-ADSC group than in the OVX group. Additionally, a histologic examination revealed new bone formation at the fusion bed between the lamina and implanted iliac crest bone in the h-ADSC group, whereas, in the OVX group, the fusion masses were composed of fibroblastic proliferation.
CONCLUSION: Our study demonstrates that the administration of h-ADSCs may have advantages in bone formation and consolidation but does not lead to bone overgrowth. These findings indicate that the administration of h-ADSCs is an alternative and efficient method for spinal fusion.
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