An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection
DC Field | Value | Language |
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dc.contributor.author | Kang, Dongkyu | - |
dc.contributor.author | Ahn, Hyun Joo | - |
dc.contributor.author | Lee, Jiho | - |
dc.contributor.author | Kim, Sang Kyung | - |
dc.contributor.author | Pyun, Jaechul | - |
dc.contributor.author | Song, Chang-Seon | - |
dc.contributor.author | Kim, Sang Jick | - |
dc.contributor.author | Lee, Joonseok | - |
dc.date.accessioned | 2022-07-06T12:02:06Z | - |
dc.date.available | 2022-07-06T12:02:06Z | - |
dc.date.created | 2021-12-08 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/140784 | - |
dc.description.abstract | Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIRabsorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self calibrating LRET systems in the background-free NIR region. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Joonseok | - |
dc.identifier.doi | 10.1016/j.bios.2021.113369 | - |
dc.identifier.scopusid | 2-s2.0-85107304431 | - |
dc.identifier.wosid | 000704123100003 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.190, pp.1 - 8 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 190 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | UP-CONVERSION LUMINESCENCE | - |
dc.subject.keywordPlus | RECENT PROGRESS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | FRET | - |
dc.subject.keywordPlus | NANOCRYSTALS | - |
dc.subject.keywordPlus | ORIENTATION | - |
dc.subject.keywordPlus | EMISSION | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Lanthanide-doped nanoparticles | - |
dc.subject.keywordAuthor | Single-chain variable fragment | - |
dc.subject.keywordAuthor | Near-infrared region | - |
dc.subject.keywordAuthor | Homogeneous sandwich-immunoassay | - |
dc.subject.keywordAuthor | Avian origin-virus | - |
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