An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection
- Authors
- Kang, Dongkyu; Ahn, Hyun Joo; Lee, Jiho; Kim, Sang Kyung; Pyun, Jaechul; Song, Chang-Seon; Kim, Sang Jick; Lee, Joonseok
- Issue Date
- Oct-2021
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Lanthanide-doped nanoparticles; Single-chain variable fragment; Near-infrared region; Homogeneous sandwich-immunoassay; Avian origin-virus
- Citation
- BIOSENSORS & BIOELECTRONICS, v.190, pp.1 - 8
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 190
- Start Page
- 1
- End Page
- 8
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/140784
- DOI
- 10.1016/j.bios.2021.113369
- ISSN
- 0956-5663
- Abstract
- Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIRabsorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self calibrating LRET systems in the background-free NIR region.
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