Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency
- Authors
- Choi, In Young; Lim, HoTae; Huynh, Alex; Schofield, James; Cho, Hyeon Jin; Lee, Hosuk; Andersen, Peter; Shin, Joo Heon; Heo, Won Do; Hyun, Sang-Hwan; Kim, Yong Jun; Oh, Yohan; Kim, Hyesoo; Lee, Gabsang
- Issue Date
- Feb-2021
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Optogenetics; FGF2; FGF signaling; Pluripotent stem cell; Pluripotency
- Citation
- BIOMATERIALS, v.269, pp.1 - 11
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMATERIALS
- Volume
- 269
- Start Page
- 1
- End Page
- 11
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/142360
- DOI
- 10.1016/j.biomaterials.2020.120222
- ISSN
- 0142-9612
- Abstract
- Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture.
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