In Vitro and Cell Imaging-Based Analysis of Protease Activity Using Nanoparticles나노입자를 활용한 In vitro 및 세포이미징 기반 단백질분해 효소활성 분석법
- Other Titles
- 나노입자를 활용한 In vitro 및 세포이미징 기반 단백질분해 효소활성 분석법
- Authors
- 김계백; 김영필
- Issue Date
- Sep-2018
- Publisher
- 한국세라믹학회
- Keywords
- nanoparticle; protease; enzyme activity; cell imaging
- Citation
- 세라미스트, v.21, no.3, pp.204 - 215
- Indexed
- OTHER
- Journal Title
- 세라미스트
- Volume
- 21
- Number
- 3
- Start Page
- 204
- End Page
- 215
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/142617
- DOI
- 10.31613/ceramist.2018.21.3.05
- ISSN
- 1226-976X
- Abstract
- Proteases are one of the most abundant classes of enzymes in living organisms and have been considered major targets for drug development. However, despite the ability to specifically cleave their substrates, many attempts to assay protease activity have generally relied upon the use of gel zymography or fluorophore-labeled peptide substrates, which is limited in rapid and multiplex analysis. Here we review the recent advances in nanoparticle (NP)-utilized assays of protease activity focused on in vitro and cell imaging-based approaches. Owing to large surface area and unprecedented physical properties of NPs, these approaches are anticipated to facilitate many applications related to protease activity-based disease diagnosis and drug discovery.
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