Semi-quantitative Estimation of Cellular SiO2 Nanoparticles Using Flow Cytometry Combined with X-ray Fluorescence Measurements
- Authors
- Choi, Seo Yeon; Yang, Nuri; Jeon, Soo Kyung; Yoon, Tae Hyun
- Issue Date
- Sep-2014
- Publisher
- WILEY-BLACKWELL
- Keywords
- flow cytometry; cellular SiO2 nanoparticles; X-ray fluorescence; nanoparticle toxicity
- Citation
- CYTOMETRY PART A, v.85A, no.9, pp.771 - 780
- Indexed
- SCIE
SCOPUS
- Journal Title
- CYTOMETRY PART A
- Volume
- 85A
- Number
- 9
- Start Page
- 771
- End Page
- 780
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143319
- DOI
- 10.1002/cyto.a.22481
- ISSN
- 1552-4922
- Abstract
- In this study, we have demonstrated feasibility of a semi-quantitative approach for the estimation of cellular SiO2 nanoparticles (NPs), which is based on the flow cytometry measurements of their normalized side scattering intensity. In order to improve our understanding on the quantitative aspects of cell-nanoparticle interactions, flow cytometry, transmission electron microscopy, and X-ray fluorescence experiments were carefully performed for the HeLa cells exposed to SiO2 NPs with different core diameters, hydrodynamic sizes, and surface charges. Based on the observed relationships among the experimental data, a semi-quantitative cellular SiO2 NPs estimation method from their normalized side scattering and core diameters was proposed, which can be applied for the determination of cellular SiO2 NPs within their size-dependent linear ranges.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 자연과학대학 > 서울 화학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.