An Image Cytometric MTT Assay as an Alternative Assessment Method of Nanoparticle Cytotoxicityopen access
- Authors
- Lee, Song Hee; Park, Jonghoon; Kwon, Dongwook; Yoon, Tae Hyun
- Issue Date
- Jul-2014
- Publisher
- KOREAN CHEMICAL SOC
- Keywords
- Alternative assay method; Nanoparticles cytotoxicity; Image cytometry; MTT assay; TiO2 nanoparticles
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.35, no.7, pp.1933 - 1938
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 35
- Number
- 7
- Start Page
- 1933
- End Page
- 1938
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143359
- DOI
- 10.5012/bkcs.2014.35.7.1933
- ISSN
- 0253-2964
- Abstract
- Despite increasing importance of in vitro cell-based assays for the assessment of nanoparticles (NPs) cytotoxicity, their suitability for the assessment of NPs toxicity is still in doubt. Here, limitations of widely used cell viability assay protocol (i.e., MTT asssay) for the cytotoxicity assessment of P25 TiO2 NPs were carefully examined and an alternative toxicity assessment method to overcome these limitations was proposed, where the artifacts caused by extracellularly formed formazan and light scattered by agglomerated NPs were minimized by measuring only the intracellular formazan via image cytometric methods.
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