Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination
- Authors
- Lim, Jun Hyung; Park, Geun Chul; Lee, Seung Muk; Lee, Jung Heon; Lim, Butaek; Hwang, Soo Min; Kim, Jung Ho; Park, Hansoo; Joo, Jinho; Kim, Young-Pil
- Issue Date
- Jul-2015
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- affinity tags; bioluminescence; metal coordination; quantum dots; resonance energy transfer; ZnO nanorods
- Citation
- SMALL, v.11, no.28, pp.3469 - 3475
- Indexed
- SCIE
SCOPUS
- Journal Title
- SMALL
- Volume
- 11
- Number
- 28
- Start Page
- 3469
- End Page
- 3475
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143438
- DOI
- 10.1002/smll.201403700
- ISSN
- 1613-6810
- Abstract
- The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy-modified quantum dot (QD) acting as a donor-acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine-tagged Luc (Luc-His 6) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD-BRET reactions with metal ions (e.g., zinc ions), a geometry-controlled ZnO NR platform can facilitate the design of surface-initiated BRET sensors without being supplemented by copious metal ions: the geometry-controlled ZnO NR platform can therefore pave the way for nanostructure-based biosensors with enhanced analytical performance.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143438)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.