Analysis of antifreeze protein activity using colorimetric gold nanosensors
- Authors
- Jing, Xu; Choi, Ho-seok; Park, Ji-In; Kim, Young-Pil
- Issue Date
- Jul-2015
- Publisher
- SPIE
- Keywords
- antifreeze protein; colorimetric analysis; gold nanoparticle; ice-binding protein; protein activity
- Citation
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v.9523
- Indexed
- SCOPUS
- Journal Title
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE
- Volume
- 9523
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143441
- DOI
- 10.1117/12.2189039
- ISSN
- 1605-7422
- Abstract
- High activity and long stability of antifreeze proteins (AFPs), also known as ice-binding proteins (IBPs), are necessary for exerting their physiological functions in biotechnology and cryomedicine. Here we report a simple analysis of antifreeze protein activity and stability based on self-Assembly of gold nanoparticles (AuNPs) via freezing and thawing cycles. While the mercaptosuccinic acid-capped AuNP (MSA-AuNP) was easily self-Assembled after a freezing/thawing cycle, due to the mechanical attack of ice crystal on the MSA-AuNP surface, the presence of AFP impeded the self-Assembly of MSA-AuNP via the interaction of AFP with ice crystals via freezing and thawing cycles, which led to a strong color in the MSA-AuNP solution. As a result, the aggregation parameter (E520/E650) of MSA-AuNP showed the rapid detection of both activity and stability of AFPs. We suggest that our newly developed method is very suitable for measuring antifreeze activity and stability in a simple and rapid manner with reliable quantification.
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