One-Pot Enzymatic Conversion of Carbon Dioxide and Utilization for Improved Microbial Growth
- Authors
- Hong, Sung-Gil; Jeon, Hancheol; Kim, Han Sol; Jun, Seung-Hyun; Jin, EonSeon; Kim, Jungbae
- Issue Date
- Apr-2015
- Publisher
- AMER CHEMICAL SOC
- Citation
- ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.49, no.7, pp.4466 - 4472
- Indexed
- SCIE
SCOPUS
- Journal Title
- ENVIRONMENTAL SCIENCE & TECHNOLOGY
- Volume
- 49
- Number
- 7
- Start Page
- 4466
- End Page
- 4472
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143563
- DOI
- 10.1021/es505143f
- ISSN
- 0013-936X
- Abstract
- We developed a process for one-pot CO2 conversion and utilization based on simple conversion of CO2 to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO2 conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO2, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO2 conversion and utilization is an alternative as well as complementary process to adsorption-based CO2 capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
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