Enzymatic Esterification under High-pressure CO₂Conditions forin situRecovery of Butyric Acid from Anaerobic Fermenters
- Authors
- Chun, Jaesung; Sang, Byoung-In
- Issue Date
- Aug-2020
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- Clostridium tyrobutyricum; butyric acid; butyl butyrate; extraction process; carbon dioxide
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.25, no.4, pp.616 - 622
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 25
- Number
- 4
- Start Page
- 616
- End Page
- 622
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145361
- DOI
- 10.1007/s12257-020-0158-7
- ISSN
- 1226-8372
- Abstract
- Butyric acid, a short chain carboxylic acid with diverse usages, is produced by Clostridia fermentation. In the industrial scale production of butyric acid, its separation and recovery from fermentation broth requires energy-intensive processes. To reduce the product recovery costs, it is necessary to convert butyric acid into a chemical with a much higher partition coefficient in the hydrophobic extractants than butyric acid. Butyl butyrate, with an excellent partition coefficient to tetradecane, can be produced by the enzymatic conversion of butyric acid via esterification. Moreover, butyl butyrate can be used as a valuable fuel source and additive in the food, cosmetic, and pharmaceutical industries. Novozyme 435,Candida antarcticalipase B immobilized on acrylic resin, and tetradecane were used as the enzyme and extractant, respectively. A high-pressure CO2-facilitated reactor was used to temporarily drop the pH of the fermentation broth so that the enzymatic reaction could be activated. Thein situremoval of butyric acid and simultaneous production of butyl butyrate were processed continuously during fermentation. To optimize the enzymatic reaction, it was necessary to maintain a temperature of 40 degrees C at 50 bar and an optimal molar ratio of substrate. In the extractive fermentation, 11.6 g/L butyl butyrate was produced with a productivity of 0.77 g/L/h from butyrate produced through fermentation. This process is expected to be able to extract carboxylic acids with more diverse carbon lengths in ester form.
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