Efficient, Simple Production of Corresponding Alcohols from Supplemented C₂-C₈Carboxylic Acids inEscherichia coliUsing Acyl-CoA Transferase fromMegasphaera hexanoica
- Authors
- Park, Hyojung; Jeon, Byoung Seung; Sang, Byoung-In
- Issue Date
- Aug-2020
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- acyl-CoA transferase; alcohol; carboxylic acid; Megasphaera hexanoica
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.25, no.4, pp.599 - 606
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 25
- Number
- 4
- Start Page
- 599
- End Page
- 606
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145362
- DOI
- 10.1007/s12257-020-0163-x
- ISSN
- 1226-8372
- Abstract
- The accumulation of short-chain carboxylic acids (SCCAs), such as acetic acid (C-2), propionic acid (C-3), butyric acid (C-4), and valeric acid (C-5), produced by acetogens in the anaerobic digestion (AD) process hampers the maintenance of stable AD processes in biomethane production. The conversion of various SCCAs to the corresponding alcohols can be a good solution not only for utilizing abandoned or harmful SCCAs and producing useful alcohols but also for increasing the efficiency of the biogas production process. ACT01_02765 (acyl-CoA transferase) fromMegasphaera hexanoicaquickly and easily converted C-2-C(8)carboxylic acids into the corresponding alcohols inEscherichia coliwith AdhE2 (alcohol dehydrogenase) fromClostridium acetobutylicum. E. coli(ACT01_02765 and AdhE2) converted carboxylic acids to the corresponding alcohols with a conversion yield that was approximately 40 times higher and a conversion rate that was approximately 10-50 times faster than those ofE. coli(Ptb-Buk and AdhE2). The enzymatic machinery inE. coli(ACT01_02765 and AdhE2) is effective for carboxylic acids of different carbon chain lengths, resulting in the production of propanol, butanol, pentanol, hexanol, heptanol, and octanol.
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